SOI nanowire transistor for detection of D-NFATc1 molecules

Ivanov, Yu. D.; Pleshakova, Tatyana O.; Kozlov, Andrey F.; Malsagova, Kristina A.; Krokhin, N. V.; Кайшева, Анна Леонидовна; Shumov, Ivan D.; Popov, V.; Naumova, O. V.; Fomin, B. I.; Nasimov, D. A.; Aseev, A. L.; Archakov, Alexander I.

doi:10.3103/s8756699013050142

Cited by 11 publications

(12 citation statements)

References 26 publications

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“…This made it possible to provide internal signal amplification due to the exponential dependence of the current in the subthreshold SOI mode of the NW sensor (transistor). Subsequently, SOI-NW sensors were used to detect HBsAg markers of hepatitis B, the liver tumor marker α-fetoprotein (sensitivity in the range of 10 –14 to 10 –15 M) [ 5 ], and the lung tumor marker D-NFATc1 with a sensitivity of 2.5 × 10 –15 M [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Biosensors Based on SOI Nanowire Transistors for Biomedicine and Virusology

et al. 2021

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This article contains the results of research on the topical problem of highly sensitive express registration of biological objects using field-effect transistors with the surface open for analyte access, which are made based on silicon-on-insulator (SOI) films. The possibilities of dielectrophoretic effects for controlling the concentration of the analyte in the area of sensory elements are considered on the example of the indication of viruses of nuclear polyhedrosis and vaccinia. It is shown that the use of the dielectrophoresis (DEPh) effect makes it possible to solve (1) the key tasks for creating sensor systems: increasing the detecting ability, as well as exrtacting and verifying the signal from the target particles; and (2) the fundamental task: determining the charge state of the analyte in solutions without modifying the sensors' surface. The problems and prospects of the mass application of nanowire (NW) biosensors, including those with the dielectrophoretic effect, in biotechnology, virology, etc., are discussed.

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Section: Introductionmentioning

confidence: 99%

Biosensors Based on SOI Nanowire Transistors for Biomedicine and Virusology

et al. 2021

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“…The fabrication and characteristics of the SOI-nanowire sensor chips (SOI-NW chips) are described in detail elsewhere [ 15 , 16 ]. The process of silicon-on-insulator (SOI) nanowire chips fabrication, as schematically shown in Figure 1 , comprised of the following steps: the production of initial SOI structures with a cut-off Si layer thickness of 500–600 nm while using hydrogen exfoliation technology; thinning of the SOI layer to nanometer dimensions by a sequential cycle of operations-thermal oxidation; removal of sacrificial oxide in HF solution; lateral structuring of the SOI layers using optical or electron lithography to form nanowire structures with contact areas; the formation of ohmic contacts to nanometer thick SOI layer by thickening the SOI in the contact areas by a poly-Si layer deposition and subsequent doping; lateral structuring of SOI layers while using electronic lithography and gas-plasma chemical etching, which allows for one to form a nanometer-size active element; metallization and contact wiring; and, finally, crystal cutting.…”

Section: Methodsmentioning

confidence: 99%

“…The surface of the SOI-NW chips was first treated chemically, in order to remove the organic contaminants and the natural oxide from the sensor surface, with isopropanol, HF, and CH 3 OH similarly to the procedure that was described in our previous papers [ 14 , 15 , 16 , 17 , 18 ]. After that, the chips were treated with glow discharge plasma in a homemade apparatus that was developed in JIHT RAS, in order to form OH groups on the sensor surface, and then the chip was treated in APTES vapors, according to [ 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…This chip was fabricated on the basis of silicon-on-insulator (SOI) structure employing complementary metal–oxide–semiconductor (CMOS)-compatible technology. In contrast to our previous studies [ 14 , 15 , 16 , 17 , 18 ], before the surface functionalization, the sensor chips were treated with glow discharge plasma instead of ozone treatment. The antibody-functionalized chips have been used for the highly sensitive detection of high molecular weight glycoprotein CA 125—a protein marker of ovarian cancer—in purified buffer solution.…”

Section: Introductionmentioning

confidence: 99%

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Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

et al. 2020

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The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10−16 M.

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“…The use of biosensors with sensor elements of nanometer size-such as nanowires and nanoribbons-opens new horizons in biomedical research. These biosensors allow one to perform direct label-free detection of various targets-such as viral particles [6] and biological macromolecules (proteins [7][8][9][10][11][12][13] and nucleic acids [14][15][16][17][18][19])-in real time, attaining low (<1 fM, i.e., <10 −15 M) concentration detection limits [20]. The principle of operation of these biosensors consists of recording a modulation of an electric current, flowing through the sensor elements, in the course of binding of target particles (i.e., macromolecules or viral particles of interest) to their surface.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Sensitized Nanoribbon Biosensor for Ovarian Cancer Marker Detection in Plasma

et al. 2021

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The detection of CA 125 protein in buffer solution with a silicon-on-insulator (SOI)-based nanoribbon (NR) biosensor was experimentally demonstrated. In the biosensor, sensor chips, bearing an array of 12 nanoribbons (NRs) with n-type conductance, were employed. In the course of the analysis with the NR biosensor, the target protein was biospecifically captured onto the surface of the NRs, which was sensitized with covalently immobilized aptamers against CA 125. Atomic force microscopy (AFM) and mass spectrometry (MS) were employed in order to confirm the formation of the probe–target complexes on the NR surface. Via AFM and MS, the formation of aptamer–antigen complexes on the surface of SOI substrates with covalently immobilized aptamers against CA 125 was revealed, thus confirming the efficient immobilization of the aptamers onto the SOI surface. The biosensor signal, resulting from the biospecific interaction between CA 125 and the NR-immobilized aptamer probes, was shown to increase with an increase in the target protein concentration. The minimum detectable CA 125 concentration was as low as 1.5 × 10−17 M. Moreover, with the biosensor proposed herein, the detection of CA 125 in the plasma of ovarian cancer patients was demonstrated.

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SOI nanowire transistor for detection of D-NFATc1 molecules

Cited by 11 publications

References 26 publications

Biosensors Based on SOI Nanowire Transistors for Biomedicine and Virusology

Biosensors Based on SOI Nanowire Transistors for Biomedicine and Virusology

Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor

Aptamer-Sensitized Nanoribbon Biosensor for Ovarian Cancer Marker Detection in Plasma

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