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Testis-specific gene antigen10 (Tsga10), as a cytoskeletal protein in the sperm tail, impacts the sperm motility. This study investigates the correlation between sperm profile alterations and Tsga10 gene expression in adult mice exposed to formaldehyde (FA) and then treated with antioxidant effect of manganese (Mn ). In this regard, we examined 35 NMRI adult male mice (6-8 weeks age) in 4 groups of control, sham, FA-exposed and FA+Mn . The mice in FA+Mn group were exposed to FA (10 mg kg twice a day) for 2 weeks and treated with daily Mn administration (5 mg kg ) in the second week prior to sacrificing the mice for testis dissection. The right testis was dissected in each group and subjected to RNA extraction and cDNA syntheses for gene expression analysis by real-time PCR. The findings revealed that FA decreased sperm parameters and Tsga10 expression (52.6 ± 24.37%). However, the injected powerful manganese antioxidant improved sperm profile through overexpression of Tsga10 (121.6 ± 27.13%) under FA-induced stressful condition which proves the correlation between sperm profile and Tsga10 expression (P ≤ 0.05). This study also shows that Tsga10 expression protects sperm dysfunction in FA+Mn group and resulting in better preservation of spermatozoa and improvement of male fertility.

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Many neurodegenerative disorders are characterized by the abnormal aggregation of misfolded proteins that form amyloid deposits which possess prion-like behavior such as self-replication, intercellular transmission, and consequent induction of native forms of the same protein in surrounding cells. The distribution of the accumulated proteins and their correlated toxicity seem to be involved in the progression of nervous system degeneration. Molecular chaperones are known to maintain proteostasis, contribute to protein refolding to protect their function, and eliminate fatally misfolded proteins, prohibiting harmful effects. However, chaperone network efficiency declines during aging, prompting the onset and the development of neurological disorders. Extracellular vesicles (EVs) are tiny membranous structures produced by a wide range of cells under physiological and pathological conditions, suggesting their significant role in fundamental processes particularly in cellular communication. They modulate the behavior of nearby and distant cells through their biological cargo. In the pathological context, EVs transport disease-causing entities, including prions, α-syn, and tau, helping to spread damage to non-affected areas and accelerating the progression of neurodegeneration. However, EVs are considered effective for delivering therapeutic factors to the nervous system, since they are capable of crossing the blood–brain barrier (BBB) and are involved in the transportation of a variety of cellular entities. Here, we review the neurodegeneration process caused mainly by the inefficiency of chaperone systems as well as EV performance in neuropathies, their potential as diagnostic biomarkers and a promising EV-based therapeutic approach.

Objectives: The aim of this study is to predict the gender among Iranian population by determining the facial and nasal indices. Methods: Four hundred healthy individuals including 200 males and 200 females (age range of 20-25 years) with normal craniofacial configuration took part in the present study. Facial and nasal variables including facial length (FL), facial width (FW), facial index (FI), nasal length (NL), nasal height (NH), nasal width (NW), and nasal index (NI) measured and SPSS 22.0 software was used for statistical analysis. Results: The mean age was 22.16±2.44 years. The mean FL, FW, FI, NL, NW, and NI were 11.53±0.592 cm, 12.54±1.41 cm, 92.67±6.24 cm, 6.02±0.53 cm, 2.78±0.25 cm, and 90.79±8.22 cm, respectively. There were significant differences in the mean value of all nasofacial parameters (P<0.05). The most prominent face and nose types were hyperleptoprosopic (46%) and platyrrhine (57.75%) among Iranian population. The highest sensitivity and specificity to predict the gender dimorphism were related to FL and FW (.990 and 0.97) and (.970 and 0.94), respectively. Conclusion:  Nasofacial parameters such as FL and FW that are able to sexual dimorphism with high sensitivity and specificity can be helpful in forensic medicine. Also, the nasofacial parameters are useful in in reconstructive, and aesthetic surgeries.