A higher concentration of airborne micro-organisms and toxic trace metals in the air is a potential risk for human and environmental health. Suspended dust and microbes in the air are the main inducers of respiratory symptoms. This study aimed to estimate and characterize the microflora and heavy metal contaminants in the car cabin filters (CAFs) dust of Lahore, Pakistan. Moreover, toxicological characterization of the dust was done using relevant cell culture of Human Lung Alveolar Epithelial Cells A-549 through MTT assay. Culturable bacteria and fungi populations were quantified using the standard spread plate method and heavy metals lead, chromium, and cadmium concentrations were checked through atomic absorption spectroscopy. Kirby-Bauer disk diffusion method was used to determine the antibiotic resistance profile of the isolated bacterial species. The mean concentration of culturable bacteria and fungi was 4X10 6 and 5X10 5 CFU/g of dust respectively.
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