Taxodium Rich is well known for its flooding tolerance and has great ecological and economic potential. A comprehensive understanding of pollen characteristics and storage capacity is important for breeding and genetic resource conservation of the genus. In this study, we observed the microsporogenesis and pollen ornamentation, studied the conditions of in vitro pollen germination, compared the difference in pollen viability of T. distichum var. distichum measured by in vitro germination and TTC staining, analyzed the change in pollen viability after different storage times and the feasibility of using stored pollen for cross breeding. Results indicated that the pollen mother cells of T. distichum var. distichum begin to enter the meiosis stage one month before the male strobilus disperse, reach metaphase 10 days after meiosis and form pollen grains three to five days after tetrad development. Pollen germination rate topped in the culture medium of 0.012% boric acid under 30 °C after 48 h, reaching 66.81%. The TTC staining demonstrated that the pollen viability of T. distichum var. distichum TD-4 and TD-5 were 97.78% and 80.54%, 98.96% and 91.67%, and 83.67% and 21.75% after one-, two- and three-year storage at −20 °C, which is significantly higher than (p < 0.05) that of 17.02 and 27.04%, 2.77% and 12.82%, and 0 determined by the in vitro cultivation. It is feasible to use pollen of T. distichum var. distichum TD-4 and TD-5 stored at −20 °C for one year for artificial hybridization, and the fruit setting rate and seed germination rate were 10.94 and 36.79%, and 11.47 and 65.76%, respectively.
Taxodium hybrid Zhongshanshan has been widely planted in the Yangtze River Basin (YRB) for soil and carbon conservation, with quantities over 50 million. The objective of this study was to determine how T. hybrid Zhongshanshan plantations affected soil physicochemical properties and bacterial community structure in the YRB, and to examine the consistency of changes by afforestation. Soils under T. Zhongshanshan plantations across six sites of the YRB were compared with soils of adjacent non-forested sites. Soil physicochemical properties and bacterial community structure were determined to clarify edaphic driving factors and reveal the effects of afforestation on bacteria. The results indicated that most soil attributes manifested improvements, e.g., total nitrogen in Jiangxi and Shanghai; available phosphorus in Hubei, Chongqing and Yunnan, exhibited the potential to maintain or ameliorate soil quality. A decrease in soil bulk density caused by plantation was also observed at the expense of soil macro-aggregates augment. Afforestation of T. Zhongshanshan plantation has habitually improved Shannon diversity and Chao1 richness, of which dominant phyla were Proteobacteria, Acidobacteria, and Actinobacteria, and increased the relative abundance of the phyla Proteobacteria and Nitrospirae, and the classes Flavobacteriia, Acidobacteria_Gp5, and Bacilli. We concluded that T. Zhongshanshan plantation can be employed to facilitate soil nutrient accumulation in the YRB, but that the degree, rate and direction of changes in soil attributes are sites dependent. It is recommended that afforestation of nutrient-depleted and less productive lands in the YRB should utilize this fast-growing species in combination with proper fertilization.
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