Microsporogenesis, Pollen Ornamentation, Viability of Stored Taxodium distichum var. distichum Pollen and Its Feasibility for Cross Breeding

Wang, Ziyang; Yin, Min; Creech, David; Yu, Chaoguang

doi:10.3390/f13050694

Cited by 3 publications

(3 citation statements)

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“…In order to detect pollen viability, the staining method was considered to be the easiest and fastest method [38,65,66]. The pollen viability of G. sinensis was assessed using four staining methods, and it varied with different methods.…”

Section: Methods For the Determination Of Pollen Viabilitymentioning

confidence: 99%

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Wang

et al. 2023

Forests

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Gleditsia sinensis Lam. (Fabaceae) is an endemic species in China, which has a wide range of ecological functions and high economic value. G. sinensis belongs to androdioecy, and the stamens of perfect flowers are aborted, meaning that a perfect flower is a functional female flower. Understanding the dynamic process of flowering and the characteristics of pollen morphology effectively determine the viability of pollen vitality, and the suitable conditions for short-term storage of pollen can provide theoretical basis and technical reference for hybrid breeding and germplasm conservation of G. sinensis. In this study, the male plants of G. sinensis in Guiyang area were used as research materials. The flowering dynamic process of male flowers was recorded through field observation. The morphology of pollen was observed and analyzed with a scanning electron microscope (SEM). The germination characteristics of pollen were studied with an in vitro germination method, and the pollen vitality was also determined using four staining methods. The effects of different storage temperatures and water contents on pollen germination rate were discussed. The results showed that the male flowers of G. sinensis had a short, single flowering period, lasting 2–3 days from the opening to the shedding. The dynamic opening process of a single flower was artificially divided into five stages. Pollen grains of G. sinensis are oblate spheroidal, tricolporate with equatorial elongated endoapertures and the sporoderm surface is reticulate. The MTT (Thiazolyl Blue Tetrazolium Bromide) staining method could accurately and quickly determine the pollen vitality of G. sinensis. The highest pollen germination rate was 65.89% ± 3.41%, and the length of the pollen tube was 3.96 mm after cultured in 15% sucrose + 100 mg/L boric acid + 20 mg/L calcium chloride for 24 h. It was necessary to collect the pollen at the big bud stage, which was conducive to improving the efficiency of pollen collection because the pollen had been mature with high pollen vitality at this stage. When it came to pollen preservation, the pollen germination rate was significantly affected by storage time, storage temperature and pollen water content. The pollen still had high vitality after being stored at −80 °C for 30 days when the moisture content of the pollen decreased to 9%, and the pollen germination rate only decreased by 28.84% compared with that before storage. In conclusion, this study has comprehensively and systematically studied the morphology, vitality determination and preservation methods of the pollen of G. sinensis, providing a theoretical basis for the cross regional breeding and the conservation and utilization of germplasm resources.

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Section: Methods For the Determination Of Pollen Viabilitymentioning

confidence: 99%

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Wang

et al. 2023

Forests

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“…Pollen is the male gametophyte that carries plant genetic information and its study is relevant since the adequate male germline development is a prerequisite for proper crops performance 38 , 39 . Pollen development is a critical step in plant development that is needed for successful breeding and seed formation 29 .…”

Section: Discussionmentioning

confidence: 99%

Morphological and anatomical characterization of yellow diploid potato flower for effective breeding program

Bohorquez-Quintero

Galvis-Tarazona

Moreno

et al. 2022

Sci Rep

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The diploid yellow potato (Solanum tuberosum L. Phureja Group) is an important plant genetic resource. In this study, we report for the first time the characterization of anther development and pollen formation in the cultivar Criolla Colombia. The description of morphological and histological characters of buds and flowers at different developmental stages permitted to identify ten main stages, from the differentiation of the male cells of the sporangium, meiosis, microspores formation and maturation, to the release of mature pollen. In addition, the results provide a graphic guide of the development of the anther, through the sequential and orderly formation of the epidermis, the endothecium, the middle layer and the nutritive layer or tapetum. This microanatomical information will be useful for work focused on androgenesis and identification of gene regulation in floral biology and gamete formation. Therefore, this study determined that to efficiently obtain haploids, flower buds between 5 and 8.9 mm long (stage 6 to 8) should be used, in which tetrads and microspores are in the early uninucleate and binucleate stage.

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“…However, the model species of the present study, T. distichum (L.) Rich, also shows extraordinary flood tolerance. It is a deciduous tree of the Taxodium genus, native to North America and Mexico where it forms large natural stands mostly in coastal plains affected by tide, in marshes with poor drainage, and in lowlands with periodic flooding ( Wang et al., 2022 ; Guo et al., 2023 ). Owing to its outstanding flood tolerance, T. distichum has broad application prospects and is promoted for use in ecosystem restoration and construction of wetlands ( Ding et al., 2021 ; He et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Response of underwater photosynthesis to light, CO2, temperature, and submergence time of Taxodium distichum, a flood-tolerant tree

Guo,

Xue,

Yin

et al. 2024

Front. Plant Sci.

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IntroductionPartial or complete submergence of trees can occur in natural wetlands during times of high waters, but the submergence events have increased in severity and frequency over the past decades. Taxodium distichum is well-known for its waterlogging tolerance, but there are also numerous observations of this species becoming partially or complete submerged for longer periods of time. Consequently, the aims of the present study were to characterize underwater net photosynthesis (PN) and leaf anatomy of T. distichum with time of submergence.MethodsWe completely submerged 6 months old seedling of T. distichum and diagnosed underwater (PN), hydrophobicity, gas film thickness, Chlorophyll concentration and needles anatomy at discrete time points during a 30-day submergence event. We also constructed response curves of underwater PN to CO2, light and temperature.ResultsDuring the 30-day submergence period, no growth or formation new leaves were observed, and therefore T. distichum shows a quiescence response to submergence. The hydrophobicity of the needles declined during the submergence event resulting in complete loss of gas films. However, the Chlorophyll concentration of the needles also declined significantly, and it was there not possible to identify the main cause of the corresponding significant decline in underwater PN. Nevertheless, even after 30 days of complete submergence, the needles still retained some capacity for underwater photosynthesis under optimal light and CO2 conditions.DiscussionHowever, to fully understand the stunning submergence tolerance of T. distichum, we propose that future research concentrate on unravelling the finer details in needle anatomy and biochemistry as these changes occur during submergence.

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Microsporogenesis, Pollen Ornamentation, Viability of Stored Taxodium distichum var. distichum Pollen and Its Feasibility for Cross Breeding

Cited by 3 publications

References 38 publications

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Studies on Pollen Morphology, Pollen Vitality and Preservation Methods of Gleditsia sinensis Lam. (Fabaceae)

Morphological and anatomical characterization of yellow diploid potato flower for effective breeding program

Response of underwater photosynthesis to light, CO2, temperature, and submergence time of Taxodium distichum, a flood-tolerant tree

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