Background aims. Adipose tissue-derived mesenchymal stromal cells (MSCs) have a higher capacity for proliferation and differentiation compared with other cell lineages. Although distraction osteogenesis is the most important therapy for treating bone defects, this treatment is restricted in many situations. The aim of this study was to examine the therapeutic potential of adipose tissue-derived MSCs and osteoblasts differentiated from adipose tissue-derived MSCs in the treatment of bone defects. Methods. Bone defects were produced in the tibias of New Zealand rabbits that had previously undergone adipose tissue extraction. Tibial osteotomy was performed, and a distractor was placed on the right leg of the rabbits. The rabbits were placed in control (group I), stem cell (group II) and osteoblast-differentiated stem cell (group III) treatment groups. The rabbits were sacrificed, and the defect area was evaluated by radiologic, biomechanical and histopathologic tests to examine the therapeutic effects of adipose tissue-derived MSCs. Results. Radiologic analyses revealed that callus density and the ossification rate increased in group III compared with group I and group II. In biomechanical tests, the highest ossification rate was observed in group III. Histopathologic studies showed that the quality of newly formed bone and the number of cells active in bone formation were significantly higher in group III rabbits compared with group I and group II rabbits. Conclusions. These data reveal that osteoblasts differentiated from adipose tissue-derived MSCs shorten the consolidation period of distraction osteogenesis. Stem cells could be used as an effective treatment for bone defects.
The herbicide itself and the degradation products are highly toxic on biological systems. The aim of this study is to investigate the potential toxic effects of trifluralin (TRF) on the urinary system of male rats and to investigate the protective effects of resveratrol (RSV) in TRF-induced urinary system damage. A total of 35 male Wistar rats were randomly divided into: (1) control group, (2) sham group, (3) low dose TRF group (0.8 g/kg/day), (4) high dose TRF group (2 g/kg/day) and (5) high dose TRF + RSV group 10 mg/kg/day. RSV was administered for 21 days by intragastric gavage at a dose of 10 mg/kg/day after induction of TRF. Kidney, ureter and urinary bladder tissue was examined using light microscopy and ultrastructurally. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling was performed to detect apoptosis. Superoxide dismutase (SOD), glutathion peroxidase (GPx) and malondialdehyde (MDA) levels were also evaluated biochemically for oxidative stress parameters. Histological evaluation showed that TRF increases apoptosis and oxidative stress, causes histological tissue damages and biochemical changes in the kidneys but does not cause any damage to the ureter and bladder. Treatment with RSV significantly attenuated tissue damage in the urinary system of rats. Apopitotic cells were significantly decreased in the treatment group. Additionally, treatment with RSV decreased SOD and GPx levels and increased MDA levels in the kidney tissue of animals subjected to TRF. These results show that RSV can significantly minimize histological damage and biochemical differences in treating TRF-induced kidney injury in rats.
abbits' higher sensitivity to infections, stronger muscles and vulnerability to emotional stress require careful and fastidious laboratory practices. Our study consisted of the installment of the Ilizarov fixator on New Zealand white rabbits' tibias and tibia extension via application of Adipose Derived Adult Stem cells (ADAS cells), of which the osteogenic differentiation was applied, to the fracture line. 1,2 We used Ilizarov distractors that were prepared to adjust to rabbit tibia (Figure 1). After a 2 cm incision was made on the anterior tibia, the extensor muscles were exposed and separated from the bone. The exposed bone was cut using a power-operated sur
Background: Anti-mullerian hormone is the most commonly used predictor for in vitro fertilization cycles outcome from the quantity point of view but not the quality.Methods: The study included retrospective analysis of 247 cycles out come up to blastocyst rate. Patients included in the study were divided in to three groups according to Anti-Mullerian hormone value.Results: The primary outcome included cycles outcome rates up to blastocyst rate, cycles without blastocyst, cycles with 100% blastocyst. While secondary outcome included oocyte number, number of mature oocyte, number of fertilized oocytes, number of cleaved oocytes, number of blastocyst. We found that the mean number of blastocyst is significantly affected by AMH in favor of group C, but the blastocyst rate was not significantly affected between the three groups group A (58.16%), group B (52.56%), group C (55.49%) with p value 0.621 which is not significant. The rate of cycles with 100% blastocyst rate was not significantly different between the groups, however the rate of cycles without blastocyst was higher in group B (13.58%) but it did not differ between the groups A (7.4%) and group C (7.9%). The difference was not significant between the three groups.Conclusions: AMH can be a good predictor of IVF cycles outcome based on the ovarian yield and numerical outcome but it is not a good predictor of the blastocyst rate which could indicate the quality of the outcome of the cycles in patients 35 years of age or less.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.