Background:
To enhance the utilization of highly bioactive silkworm pupae protein hydrolysate, a novel silkworm pupae protein hydrolysate-calcium chelate was prepared, which enhanced the antioxidant activity and calcium bioavailability.
background:
As the population ages, the problem of calcium deficiency among the elderly is becoming more and more serious. The most serious problem caused by calcium deficiency is osteoporosis, which induces a series of diseases.
Method:
Silkworm pupae protein hydrolysate was prepared by Alcalase hydrolysis and ultrafiltration membranes with a molecular weight cutoff of 5 kDa. The optimal process conditions for preparing peptide calcium chelate were the peptide calcium mass ratio of 2.99:1, pH of 6.72, a temperature of 35.57°C, and time of 32.79 min by Box-Behnken design, and the calcium-chelating rate reached 84.67%. The DPPH radical scavenging activity of silkworm pupae protein hydrolysate-calcium chelate was 79.36±4.31%, significantly higher than silkworm pupae protein hydrolysate (61.00±9.56%). Fourier transform infrared spectroscopy shows that the COO-, N-H, C-H, and C-O groups participated in the formation of silkworm pupae protein hydrolysate-calcium chelate. The particle size of the silkworm pupae protein hydrolysate-calcium chelate was 970.75±30.12 nm, which was significantly higher than that of silkworm pupae protein hydrolysate (253.14±5.72 nm).
Results:
The silkworm pupae protein hydrolysate-calcium chelate showed a calcium dissolution rate of 71.01±1.91% in the simulated intestinal phase, significantly higher than that of CaCl2 (59.34±1.24%). In the Caco-2 cell monolayers, the silkworm pupae protein hydrolysate-calcium chelate was more favorable for calcium transport.
method:
Silkworm pupae protein was hydrolyzed by Alcalase. SPPH-calcium chelate was prepared and characterized. The bioavailability of chelates was explored by simulating gastrointestinal digestion and Caco-2 cell absorption models.
Conclusion:
The results indicated a theoretical basis for developing novel calcium supplements for the high-value utilization of silkworm pupae.
conclusion:
In the simulated intestinal phase, the calcium solubility of the SPPH-calcium chelate was significantly higher than that of the control, which indicates that the chelate was more conducive to the digestion of calcium. In the Caco-2 cell monolayers, the SPPH-calcium chelate was more favorable for calcium transport and uptake.
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