Ultrasonic technology was applied for phenolic compounds extraction from the Pinus koraiensis bark (PKB) and response surface methodology (RSM) was used to optimize the effects of processing parameters on phenolic compounds yields. When ultrasonic power 160W,three independent variables were ultrasonic extraction time (X1 20-40min), ratio of water to raw material (X2 1:20-1:40 g/mL)and ethanol concentration(X3 20%-60%), respectively.The optimal extraction conditions of phenolic compounds were determined as follows: Ultrasonic extraction time 30.61 min, ratio of water to raw material 30.79 mL/g and ethanol concentration 42.2%. Under these conditions, the experimental yield of phenolic compounds was 8.2409 ± 0.0648‰, well matched with the predicted models with the coefficients of determination (R2) of 0.9920, which was agreed closely with the predicted value (8.2963‰).
This paper reports on a water-soluble acid polysaccharide (AAP) and in how it was extracted from Auricularia auricular, acquired by CTAB, and prepared it’s carboxymethylation. Chemical characterization by high-performance liquid chromatography/gel permeation chromatograph (HPLC/GPC), Fourier transform infrared (FT-IR) spectrometer and gas chromatography–mass spectrophotometer (GC–MS) were investigated. Chemical analysis indicated that C AAAP was composed of arabinose, xylose, mannose, glucose and galactose, with the molar ratio at 0.04: 0.13: 1.00: 0.59: 0.29. Moreover, radiation protection against UVB in vitro indicated that at the dose range of 200–500 μg/mL, C AAAP enhanced the protection of HepG2 cells against UVB cytotoxicity than AAAP. However, but at the dose range of 50–150 μg/mL the result was just opposite.
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