BackgroundLitchi is an evergreen woody tree widely cultivated in subtropical and tropical regions. Defective flowering is a major challenge for litchi production in time of climate change and global warming. Previous studies have shown that high temperature conditions encourage the growth of rudimentary leaves in panicles and suppress litchi flowering, while reactive oxygen species (ROS) generated by methyl viologen dichloride hydrate (MV) promote flowering and abortion of rudimentary leaves. To understand the molecular function of the ROS-induced abortion of rudimentary leaves in litchi, we sequenced and de novo assembled the litchi transcriptome.ResultsOur assembly encompassed 82,036 unigenes with a mean size of 710 bp, and over 58% (47,596) of unigenes showed significant similarities to known sequences in GenBank non-redundant (nr) protein database. 5,865 unigenes were found to be differentially expressed between ROS-treated and un-treated rudimentary leaves, and genes encoding signaling components of plant hormones such as ABA and ethylene were significantly enriched.ConclusionOur transcriptome data represents the comprehensive collection of expressed sequence tags (ESTs) of litchi leaves, which is a vital resource for future studies on the genomics of litchi and other closely related species. The identified differentially expressed genes also provided potential candidates for functional analysis of genes involved in litchi flowering underlying the control of rudimentary leaves in the panicles.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-805) contains supplementary material, which is available to authorized users.
Apoptosis is a form of cell death that involves the changes of mitochondrial function and the regulated activation of caspase cascades, which selectively cleave cytoskeleton proteins and catalyze the changes of cell organelles and morphological structure. The changes of mitochondrial function, cell morphological structure, and degradation of cytoskeleton are considered to be responsible for the development of meat qualities. The LM, semitendinosus, and psoas minor (PM) muscles of 5 crossbred bulls were used to observe the morphologic and quantitative changes of apoptosis, as well as the change of caspase-3 activity during 7 d storage. Transmission electron microscopy shows that the typical features of apoptosis appeared in muscles between d 1 and 4. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) positive nuclei were detected at d 4 and increased subsequently. The count of TUNEL-positive nuclei was different in 3 muscles at d 7 (P < 0.001). There was a significant increase in caspase-3 activities at 4 h postmortem relative to the activities at the first 30 min in 3 muscles (P = 0.0147 in LM; P = 0.0058 in PM; P = 0.0306 in semitendinosus), and the apexes had 2.9 to 6.5 times more activities than activities at the first 30 min postmortem. Apoptosis did exist in 3 types of muscles during the conditioning period. Apoptosis and caspase cascades system could be associated with the postmortem development of meat quality in skeletal muscles of bulls.
Cytoplasmic male sterility (CMS), widely used in the production of hybrid seeds, is a maternally inherited trait resulting in a failure to produce functional pollen. In order to identify some specific proteins associated with CMS in pepper, two-dimensional gel electrophoresis (2-DE) was applied to proteomic analysis of anthers/buds between a CMS line (designated NA3) and its maintainer (designated NB3) in Capsicum annuum L. Thirty-three spots showed more than 1.5-fold in either CMS or its maintainer. Based on mass spectrometry, 27 spots representing 23 distinct proteins in these 33 spots were identified. Proteins down-regulated in CMS anthers/buds includes ATP synthase D chain, formate dehydrogenase, alpha-mannosidas, RuBisCO large subunit-binding protein subunit beta, chloroplast manganese stabilizing protein-II, glutathione S-transferase, adenosine kinase isoform 1T-like protein, putative DNA repair protein RAD23-4, putative caffeoyl-CoA 3-O-methyltransferase, glutamine synthetase (GS), annexin Cap32, glutelin, allene oxide cyclase, etc. In CMS anthers/buds, polyphenol oxidase, ATP synthase subunit beta, and actin are up-regulated. It was predicted that male sterility in NA3 might be related to energy metabolism turbulence, excessive ethylene synthesis, and suffocation of starch synthesis. The present study lays a foundation for future investigations of gene functions associated with pollen development and cytoplasmic male sterility, and explores the molecular mechanism of CMS in pepper.
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