IPA1 gene controlled paddy has increased in height, sturdy stem, number of tillers decreased, grains per panicle and dry grains weight increased significantly. In this study, the homology of IPA1 gene in 26 types of plant from 15 families was analyzed, and thus constructed its phylogenetic tree. The results of phylogenetic tree construction based on its amino acid sequence showed that the species from the same family, such as Gramineae, Rosaceae and Palmaceae, were well clustered for different branches and had high support rates. Furthermore, we constructed the phylogenetic tree based on its CDS sequence, and the species of Gramineae was well clustered, and the support rate was 100%. Our results indicate that IPA1 has high homology in higher class of plants, especially in the Gramineae, which is of great significance for us to further study the yield of rice and other gramineous species.s with time and concentration effect (3) Achilla glauca extract could make the chromosome bridge, chromosome fragment, chromosome lag in the root tip cells of Vicia faba and increase in micronucleus rate. The results showed that the water extract of Solidago canadensis had different degree of inhibition and damage to silkworm root tip cells; it also had some genetic toxicity and rapid diffusion ability.
Botrytis cinerea is a major disease in crop production. Chrysanthemum gray mold is easy to occur during chrysanthemum growing season. It can cause a lot of defoliation when serious, affecting plant flowering and reducing ornamental value. In this study, Arabidopsis thaliana ESB1 gene introduced into Agrobacterium tumefaciens was introduced into Agrobacterium tumefaciens and its identification after induction, and the sensitivity of chrysanthemum to antibiotics. It is expected that Agrobacterium tumefaciens will be transformed into chrysanthemum, result in transgenic chrysanthemum new lines resistance to disease, high quality, and high yield to lay the technical foundation. In this study, Arabidopsis thaliana ESB1 gene was introduced into Agrobacterium tumefaciens by freeze-thawing method. The PCR reaction was used to determine whether the ESB1 gene was successfully transferred into Agrobacterium tumefaciens and the susceptibility test of chrysanthemum to hygromycin was carried out to determine the critical tolerance concentration, Establishment of Chrysanthemum Transformation Receptor System. The result of PCR showed that the ESB1 gene was successfully transferred into Agrobacterium tumefaciens. The critical concentration of chrysanthemum to hygromycin was 30mg / L.rapid diffusion ability.
Solidago decurrens is a plant of Asteraceae, which is mainly produced in southern China. It is widely used cut flower material. It is known as the malignant weed Solidago canadensis (Solidago canadensis) with the genus Flora, so its application of ecological security has been a big debate. In this paper, the allelopathic effect of the water extract of Solidago macrophylla was studied by using the micronucleus technique of Vicia faba root tip as the experimental material. The aim of this study was to analyze the genetic toxicity of Solidago canadensis. The results showed that: (1) The water extract of Solidago canadensis could decrease the mitotic index and interfere with the normal process of mitosis, where the decreased rate was positively correlated with the concentration of water extract and treatment time. (2) The effects of water extract on the root tip of Vicia faba were significant increases with time and concentration effect (3) Achilla glauca extract could make the chromosome bridge, chromosome fragment, chromosome lag in the root tip cells of Vicia faba and increase in micronucleus rate. The results showed that the water extract of Solidago canadensis had different degree of inhibition and damage to silkworm root tip cells; it also had some genetic toxicity and rapid diffusion ability.
Abstract:In this study, the actin A3 promoter was inserted into piggyBac and constructed into pBacA3, which was inserted into piggyBac. The promoter was composed of the promoter and nerve-specific 3xP3 promoter, the enhanced green fluorescent protein (EGFP) gene and the SV40 polyadenylation recognition sequence. EGFP and pBac3xP3 EGFP transposon were injected into the early fertilized eggs of Spodoptera litura to detect whether they were expressed. The results showed that both vectors could be expressed in moth eggs, and positive individuals were obtained. The results showed that the expression rate of pBacA3 EGFP was higher than that of pBac3xP3 EGFP, and the proportion of EGFP in hatching larvae was higher than that in the latter. The results showed that pBacA3 EGFP was more suitable for Spodoptera litura Transposable carrier. In vitro transient expression of the transgenic vector of Spodoptera litura was not only the first step necessary to successfully carry out the transgenic gene of Spodoptera litura, but also itself can be applied to the study of gene function, which laid the foundation for the genome research of Spodoptera litura.
The experimental object of this study is Gallus gallus (chicken) CD8a gene. In this study, we performed sequence analysis of CD8a gene and analyzed the primary structure, secondary structure and tertiary structure of CD8a encoded protein by using bioinformatics. Through the analysis, we concluded that the sequence length is 648bp with various restriction sites identified, which is similar to CD8a of other species in Galliformes order. The sequence encodes a total of 216 amino acids. The amino acids have low hydrophobicity, with 12 phosphorylation sites and a transmembrane region, and no signal peptide. The protein secondary structure mainly consists ofαspiral, accounting for 22.69%; extension chain, accounting for 15.74%; random coil, accounting for 61.57 %, in non-continuous distribution. The relationship between CD8a and other species homologous sequences is basically consistent with the establishment of the system tree by the establishment of three trees. n the root tip cells of Vicia faba and increase in micronucleus rate. The results showed that the water extract of Solidago canadensis had different degree of inhibition and damage to silkworm root tip cells; it also had some genetic toxicity and rapid diffusion ability.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.