Homology bioinformatics analysis of IPA1 gene in higher plants

Abstract

IPA1 gene controlled paddy has increased in height, sturdy stem, number of tillers decreased, grains per panicle and dry grains weight increased significantly. In this study, the homology of IPA1 gene in 26 types of plant from 15 families was analyzed, and thus constructed its phylogenetic tree. The results of phylogenetic tree construction based on its amino acid sequence showed that the species from the same family, such as Gramineae, Rosaceae and Palmaceae, were well clustered for different branches and had high support rates. Furthermore, we constructed the phylogenetic tree based on its CDS sequence, and the species of Gramineae was well clustered, and the support rate was 100%. Our results indicate that IPA1 has high homology in higher class of plants, especially in the Gramineae, which is of great significance for us to further study the yield of rice and other gramineous species.s with time and concentration effect (3) Achilla glauca extract could make the chromosome bridge, chromosome fragment, chromosome lag in the root tip cells of Vicia faba and increase in micronucleus rate. The results showed that the water extract of Solidago canadensis had different degree of inhibition and damage to silkworm root tip cells; it also had some genetic toxicity and rapid diffusion ability.

The CaALAD Gene From Pepper (Capsicum annuum L.) Confers Chilling Stress Tolerance in Transgenic Arabidopsis Plants

“…In our study, we found that transgenic plants overexpressing CaALAD grew better under low temperature stress, with more and longer roots, indicating that the CaALAD protein alleviates the inhibition of low temperature on the growth of Arabidopsis seedlings (Figure 3). By analyzing the amino acid sequence of CaALAD and comparing it with homologous proteins from other plant species, we found that ALAD is a conserved gene, which is consistent with the results of Long et al (2013). In addition, we found that it is most similar to the protein from eggplant, a related species in the Solanaceae family.…”

“…The ALAD protein plays an important role in higher organisms, and the enzymatic activity of ALAD determines the metabolism of ALA in plants, thus affecting the synthesis of tetrapyrrole molecules, especially chlorophyll (Long et al, 2013). Studies have shown that ALA plays an important role in alleviating abiotic stress, including low temperature stress (Wu et al, 2018b).…”

“…Also, ALAD activity strongly depends on the binding of metal ions (including Zn 2+ , Mn 2+ , and Mg 2+ ). In vitro assays of ALAD enzyme activity found that Ca 2+ , Mn 2+ , and K + significantly increase activity, while Cu 2+ significantly inhibits the activity of ALAD (Long et al, 2013). In cucumber, it was found that Al 3+ can significantly inhibit ALAD activity and growth in cucumber.…”

“…5-aminolevulinate dehydratase (ALAD) is a necessary enzyme for the biosynthesis of tetrapyrroles, and it catalyzes the condensation of two molecules of ALA to form porphobilinogen (PBG; Yang, 2008). Therefore, the activity of ALAD directly determines the metabolism and accumulation of ALA in plants (Long et al, 2013). Moreover, ALA does not generally accumulate in developing leaves, but is directly transformed into PBG by ALAD (Killiny et al, 2018).…”