Without any sample pretreatment, mass spectral fingerprints of 486 dried sea cucumber slices were rapidly recorded in the mass range of m/z 50-800 by using surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS). A set of 162 individual sea cucumbers (Apostichopus japonicus Selenka) grown up in 3 different geographical regions (Weihai: 59 individuals, 177 slices; Yantai: 53 individuals, 159 slices; Dalian: 50 individuals, 150 slices;) in north China sea were successfully differentiated according to their habitats both by Principal Components Analysis (PCA) and Soft Independent Modeling of Class Analogy (SIMCA) of the mass spectral raw data, demonstrating that DAPCI-MS is a practically convenient tool for high-throughput differentiation of sea cucumber products. It has been found that the difference between the body wall tissue and the epidermal tissue is heavily dependent on the habitats. The experimental data also show that the roughness of the sample surface contributes to the variance of the signal levels in a certain extent, but such variance does not fail the differentiation of the dried sea cucumber samples.
SUMMARYA method that estimates mass and stiffness matrices of shear building from modal test data is presented in this paper. The method depends on only measurable points that are less in number than the total structural degrees of freedom, and on the first two orders of structural mode measured. So it is applicable to most of the general test. Based on this method modal data of unmeasurable points are estimated, then global mass and stiffness matrices of structure are obtained by using the first two orders of modal data. Taking advantage of iteration the optimum global mass and stiffness matrices are gained. Finally, an example is studied in this paper. Its result shows that this method is reliable.1998 John Wiley & Sons, Ltd.
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