With the continued development of thermoresponsive colloidal hydrogel particles, a number of groups have begun to exploit their properties to create dynamic materials self-assembled from those components. The fundamental details of how those building blocks are assembled, the component functionality, and the geometry or length-scales present in the assemblies contribute to the behavior of the resultant material. In this tutorial review, we examine recent progress in the assembly of responsive hydrogel colloids in two and three dimensions, highlighting their potential applications, especially in the domain of biotechnology.
Microgels are colloidally stable, hydrogel microparticles that have previously been used in a range of (soft) material applications due to their tunable mechanical and chemical properties. Most commonly, thermo and pH-responsive poly(N-isopropylacrylamide) (pNIPAm) microgels can be fabricated by precipitation polymerization in the presence of the co-monomer acrylic acid (AAc). Traditionally pNIPAm microgels are synthesized in the presence of a crosslinking agent, such as N,N'-methylenebisacrylamide (BIS), however, microgels can also be synthesized under 'crosslinker free' conditions. The resulting particles have extremely low (<0.5%), core-localized crosslinking resulting from rare chain transfer reactions. AFM nanoindentation of these ultralow crosslinked (ULC) particles indicate that they are soft relative to crosslinked microgels, with a Young's modulus of ∼10 kPa. Furthermore, ULC microgels are highly deformable as indicated by a high degree of spreading on glass surfaces and the ability to translocate through nanopores significantly smaller than the hydrodynamic diameter of the particles. The size and charge of ULCs can be easily modulated by altering reaction conditions, such as temperature, monomer, surfactant and initiator concentrations, and through the addition of co-monomers. Microgels based on the widely utilized, biocompatible polymer polyethylene glycol (PEG) can also be synthesized under crosslinker free conditions. Due to their softness and deformability, ULC microgels are a unique base material for a wide variety of biomedical applications including biomaterials for drug delivery and regenerative medicine.
The equilibrium phase behavior and the dynamics of colloidal assemblies composed of soft, spherical, colloidal particles with attractive pair potentials have been studied by digital video microscopy. The particles were synthesized by precipitation copolymerization of N-isopropylacrylamide (NIPAm), acrylic acid (AAc), and N,N'-methylene bis(acrylamide) (BIS), yielding highly water swollen hydrogel microparticles (microgels) with temperature- and pH-tunable swelling properties. It is observed that in a pH = 3.0 buffer with an ionic strength of 10 mM, assemblies of pNIPAm-AAc microgels crystallize due to a delicate balance between weak attractive and soft repulsive forces. The attractive interactions are further confirmed by measurements of the crystal melting temperatures. As the temperature of colloidal crystals is increased, the crystalline phase does not melt until the temperature is far above the lower critical solution temperature (LCST) of the microgels, in stark contrast to what is typically observed for phases formed due to purely repulsive interactions. The unusual thermal stability of pNIPAm-AAc colloidal crystals demonstrates an enthalpic origin of crystallization for these microgels.
We report direct measurements of the pairwise interparticle potential between poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-coAAc) colloidal microgels as a function of pH, as determined through Ornstein-Zernike analysis of the pair distribution function of quasi-2D dilute colloidal suspensions. The interaction potential ranges from purely repulsive at high pH due to electrosteric interactions to weakly attractive at low pH due to hydrogen bonding, which explains previous observations on the unique phase behavior of these particles in concentrated suspensions.Colloidal suspensions display various phases and microscopic structures, ranging from random disordered to self-assembled ordered structures. 1 The phase behavior is determined by the effective volume fraction of the suspension and interparticle forces, such as van der Waals attractions, Coulombic interactions, steric repulsions and hydrogen-bonding. Understanding the interplay between these intermolecular forces is essential to control colloidal phase behavior, for example to fabricate ordered colloidal structures for specific applications. The majority of studies to date have been devoted to hard-sphere systems, which have simple intermolecular forces dominated by entropic excluded volume effects 2 and to other colloidal systems with relatively well-understood additional interactions, such as Coulombic repulsion and depletion attractions. 3 Only fairly recently, soft and deformable particles, in particular star polymers and microgels, have attracted significant attention under the impulse of improved synthesis techniques. These soft colloidal systems are characterized by tunability of their softness and complex interparticle interactions, which result in rich phase diagrams and unique hydrodynamic behavior. 4,5 In particular, poly(N-isopropylacrylamide) (pNIPAm)-based microgels have been studied extensively because of their reversible temperature-responsiveness. 6,7 By incorporating ionizable functional groups, e.g. acrylic acid (AAc), as co-monomers, one can synthesize ionic microgels that respond not only to temperature, but also to changes in pH and ionic strength. 8 In spite of several studies on the phase behavior of colloidal microgels as a model system for soft spheres, both from our groups and from other researchers, 7,9-12 direct measurements of the underlying interparticle interactions have not been reported. However, previous experimental observations in our groups have indicated that these interactions are non-trivial, especially in pNIPAm-co-AAc microgels. While the phase behavior of pure pNIPAm microgels can be explained relatively well by defining an effective volume fraction of particles and using hard-sphere-like interactions, the incorporation of AAc adds significant complexity to the system. Hard sphere theory is not sufficient to explain the dynamics of these pH-responsive microgels, as illustrated by the following examples from our own research. First, pNIPAm microgel suspensions are liquid-like below the hard sphere freezing volu...
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