Thermoresponsive microgel-based materials

Lyon, L. Andrew; Meng, Zhiyong; Singh, Neetu; Sorrell, Courtney D.; John, Ashlee St.

doi:10.1039/b715522k

Cited by 277 publications

(245 citation statements)

References 68 publications

(89 reference statements)

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“…26,27 In addition, in recent years, a number of different monomers showing thermoresponsive properties have been explored with the aim of increasing the range of temperatures in which these materials can operate and thus the number of different applications. 28 Where dual functionalities have been investigated, the approach has commonly involved the introduction of pH responsive monomers, such as acrylic acid (AAc), methacrylic acid or 4-vinylpyridine.…”

Section: Introductionmentioning

confidence: 99%

An l-proline based thermoresponsive and pH-switchable nanogel as a drug delivery vehicle

Salinas

Resmini

2018

Polym. Chem.

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The synthesis and characterisation of a novel dual stimuli-responsive nanogel, based on thermoresponsive N-n-propylacrylamide and an L-proline based monomer acting as a pH-switcher, is reported here. The effect of the crosslinker/co-monomer ratios was studied to demonstrate the relationship between the chemical structure, degree of hydrophobicity and physico-chemical characteristics of the nanogels. Tailoring of the thermoresponsive properties was achieved by altering crosslinker N,N'-methylenebis (acrylamide) content between 10 and 50 mol%, in combination with three thermoresponsive monomers N-n-isopropylacrylamide, N-n-propylacrylamide and N-acryloylpyrrolidine. A library of 25 different combinations of monomers and crosslinkers was obtained and characterised by dynamic light scattering and UV-Vis spectroscopy. The nanogel based on N-n-propylacrylamide and 10 mol% crosslinker was then co-polymerised with an L-proline based monomer to introduce a pH-switch. This nanogel was obtained with <10 nm particle size and a VPTT ca. 43°C, and was used to demonstrate a drug delivery capability using Nile Blue A as a model drug. Detailed studies demonstrated maximum drug release at lower pH and 43°C, thus confirming the dual stimuli switch.

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Section: Introductionmentioning

confidence: 99%

An l-proline based thermoresponsive and pH-switchable nanogel as a drug delivery vehicle

Salinas

Resmini

2018

Polym. Chem.

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“…Poly(N-isopropylacrylamide) (poly(NIPAm)) is a thermosensitive polymer that has been extensively studied for use in biomedical applications such as tissue engineering, drug delivery, and injectable hydrogels (26)(27)(28)(29), as it exhibits a physiologically relevant lower volume phase transition temperature (VPTT) around 33°C (30)(31)(32)(33). This temperature sensitivity makes crosslinked poly(NIPAm) nanoparticles ideal in vivo drug carriers as they are soluble in aqueous solutions at temperatures below the VPTT, allowing for efficient loading of water-soluble therapeutics via passive diffusion (31,34).…”

Section: Introductionmentioning

confidence: 99%

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

McMasters

Panitch

2015

AAPS J

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ABSTRACT. Peripheral artery disease is an atherosclerotic occlusion in the peripheral vasculature that is typically treated via percutaneous transluminal angioplasty. Unfortunately, deployment of the angioplasty balloon damages the endothelial layer, exposing the underlying collagen and allowing for the binding and activation of circulating platelets, which initiate an inflammatory cascade leading to eventual restenosis. Here, we report on the development of poly(NIPAm-MBA-AMPS-AAc) nanoparticles that have a collagen I-binding peptide crosslinked to their surface allowing them to bind to exposed collagen. Once bound, these particles mask the exposed collagen from circulating platelets, effectively reducing collagen-mediated platelet activation. Using collagen I-coated plates, we demonstrate that these particles are able to bind to collagen at concentrations above 0.5 mg/mL. Once bound, these particles inhibit collagen-mediated platelet activation by over 60%. Using light scattering and zeta potential measurements, we investigated the potential of the nanoparticles as a drug delivery platform. We have verified that the collagen-binding nanoparticles retain the temperature sensitivity common to poly(NIPAm)-based nanoparticles while remaining colloidally stable in aqueous environments. We also demonstrate that they are able to passively load and release anti-inflammatory cell penetrating peptides. Combined, we have developed a collagen-binding nanoparticle that has dual therapy potential, preventing collagen-mediated platelet activation while delivering water-soluble therapeutics directly to the damaged area.

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“…A variety of stimuli-responsive polymers whose properties change in response to temperature, pH, ionic strength, light, and chemical stress have been investigated [13][14][15][16][17]. Thermo-responsive polymers have been applied in the fields of sensors, catalyst supports, drug carriers, and bio-separation [18][19][20]. Thermo-responsive polymers based on N-isopropylacrylamide (NIPA) were the most common used in these years.…”

Section: Introductionmentioning

confidence: 99%

Thermo-Responsive Fluorescent Polymers with Diverse LCSTs for Ratiometric Temperature Sensing through FRET

Ding¹,

Wang²,

Feng³

et al. 2018

Polymers

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Abstract:Temperature is a significant parameter to regulate biological reactions and functions inside cells. Sensing the intracellular temperature with a competent method is necessary to understand life science. In this work, an energy-transfer polymeric thermometer was designed for temperature sensing. The thermometer was prepared from two thermo-responsive polymers with different lower critical solution temperatures (LCSTs) of 31.1 • C and 48.6 • C, coupling with blue and red fluorescent molecules, respectively, developed for ratiometric temperature sensing based on the Förster resonance energy transfer (FRET) mechanism. The polymers were synthesized from two monomers, N-isopropylacrylamide (NIPA) and N-isopropylmethacrylamide (NIPmA), which provided different temperature responses. The fluorescent intensity of each polymer (peaked at 436 and 628 nm, respectively) decreased upon the heating of the polymer aqueous solution. While these two polymer aqueous solutions were mixed, the fluorescent intensity decrease at 436 nm and substantial fluorescence enhancement at 628 nm was observed with the increasing temperature due to FRET effect. The cell imaging of HeLa cells by these thermo-responsive polymers was explored. The difference of LCSTs resulting in ratiometric fluorescence change would have a potential impact on the various biomedical applications.

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Thermoresponsive microgel-based materials

Cited by 277 publications

References 68 publications

An l-proline based thermoresponsive and pH-switchable nanogel as a drug delivery vehicle

An l-proline based thermoresponsive and pH-switchable nanogel as a drug delivery vehicle

Prevention of Collagen-Induced Platelet Binding and Activation by Thermosensitive Nanoparticles

Thermo-Responsive Fluorescent Polymers with Diverse LCSTs for Ratiometric Temperature Sensing through FRET

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