Background It is very important to dynamically evaluate the functional outcome in the knee after anterior cruciate ligament (ACL) reconstruction under physiological weight bearing. The objective of the current study is that we would like to compare the patellofemoral joint kinematics in three ACL status: ACL intact, ACL deficiency, ACL reconstruction. Methods Twenty patients with unilateral ACL deficient knees were recruited as preoperative group. Six months after ACL reconstruction, these ten subjects were included as postoperative subjects. Ten normal subjects with healthy knees as the control group. Each subject was asked to walk up a custom set of stairs and a single-plane fluoroscopic imaging system was used to determine the 6DOF kinematics of the injured knees, ACL reconstructed knees, and intact knees. Results ACL deficient knees showed reduced patellar flexion angle and reduced distal patellar translation during knee flexion. ACL reconstructed knees showed abnormal patellofemoral joint kinematics compared to ACL intact and ACL deficient knees, exhibiting increased patellar external rotation, lateral tilt, lateral translation during knee flexion. Conclusion These findings imply that some alterations persist after ACL deficiency and ACL reconstruction. These abnormal changes will be the onset of degeneration in patellofemoral joint even if the ACL is reconstructed in a way that restores the clinical anteroposterior stability of the knee. Some biomechanical changes should be made to improve the outcome of intervention especially in surgical treatment like ACL reconstruction.

Background: It has been demonstrated that fucosterol induces a therapeutic effect on cancer. However, the molecular mechanisms underlying the effects of fucosterol in the treatment of non-small cell lung cancer are still unclear.Methods: In this study, pharmMapper and GeneCards databases were utilized to gather the prediction of fucosterol targets and NSCLC-related targets. The mechanisms of fucosterol against NSCLC were identified in DAVID6.8 by enrichment analysis of GO and KEGG, and protein-protein interaction data was obtained from Sting Database. Molecular docking was used to predict the docking of GRB2. Moreover, the relationship of GRB2 expression and immune infiltrates was analyzed by TIMER database.Results: The results suggest that fucosterol acts against by candidate targets, such as MAPK1, EGFR, GRB2, IGF2, MAPK8 and SRC, which regulate biological processes including negative regulation of apoptotic process, peptidyl-tyrosine phosphorylation, positive regulation of cell proliferation. The Raf / MEK / ERK signaling pathway initiated by GRB2 maybe the most significant pathway for fucosterol to treat NSCLC.Conclusions: These results show that GRB2 is the key target for fucosterol in the treatment of NSCLC, which laying a theoretical foundation for further research and providing scientific support for the development of new drugs.