Bone mesenchymal stem cell‐derived exosome (BMSC‐exosome) is a potential candidate for lung ischemia–reperfusion injury (LIRI) treatment. This study aims to investigate the anti‐pyroptosis effect of BMSC‐exosomes in LIRI. The LIRI cell model was established by hypoxia/reoxygenation (H/R) treatment. Interleukin (IL)‐1β and IL‐18 levels were examined by enzyme‐linked immunosorbent assay. Cell viability was assessed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide assay. Lactate dehydrogenase (LDH) release was examined using a LDH assay kit. The interaction between microRNA (miR)‐202‐5p and cytidine monophosphate kinase 2 (CMPK2) was analyzed using dual‐luciferase reporter assay and RNA immunoprecipitation. BMSC‐exosomes promoted cell viability and suppressed pyroptosis in H/R‐treated mouse lung epithelial. miR‐202‐5p was enriched in BMSC‐exosomes, and exosomal miR‐202‐5p inhibition upregulated pyroptosis‐associated proteins, including cleaved N‐terminal Gasdermin D, nucleotide‐binding domain‐like receptor family member pyrin domain‐containing protein 3, and Caspase1. Meanwhile, miR‐202‐5p suppressed CMPK2 expression by directly targeting CMPK2. Expectedly, CMPK2 knockdown reversed the promoting effect of exosomal miR‐202‐5p inhibition on pyroptosis in LIRI. Therefore, BMSC‐derived exosome miR‐202‐5p repressed pyroptosis to inhibit LIRI progression by targeting CMPK2.
In this research, glutamine (GLN) and L-Tyrosine were synthesized into AL-GLN synthetic biomaterials to explore their protective effect on the myocardium of sepsis symptoms. In the process of preparation, acyl chloride method was used to generate, which was generated by acylation reaction
and ammonolysis reaction. In the ammonolysis reaction, the original ammonia water reaction was replaced by the ammonia water/dimethylformamide (DMF) system. The biomaterial was characterized, and it was confirmed that the product recovery rate could be maximized by adjusting the pH value to
9 during crystallization stage, and the purity could be increased from 94.23% to 98.45% after some technological improvement. During the experiment of myocardial protection in sepsis, 60 SPF rats were selected for the experiment and divided into three groups, namely the unmodified surgery
group, the GLN group and the AL-GLN group. Rats with sepsis were obtained by cecum ligation and perforation. The myocardial apoptosis index and the gene expression of Bcl-2/p53mRNA were detected at 6 h, 12 h and 24 h after operation in different groups. The results showed that the expression
of Bcl-2mRNA in the surgical group was significantly down-regulated compared with that in the surgical group (P < 0.05), the expression of Bcl-2mRNA in the GLN group was significantly increased compared with that in the surgical group (P < 0.05), and the expression of p53mRNA
in the AL-GLN group was first decreased and then slowly increased compared with that in the surgical group (P < 0.05).
Objective. To research the impact and mechanism of endothelin receptor A inhibitor BQ-123 combined with electroacupuncture on tibia cancer pain in rats. Methods. Sprague-Dawley (SD) rats were randomly divided into sham group (SHAM group) and bone cancer pain model group (BCP group). The behavior of SD rats was measured. The histology of the right tibia was observed by hematoxylin-eosin (HE) staining. The remaining rats were randomly divided into model, BQ-123, electroacupuncture, and BQ-123+ electroacupuncture group. Behavioral tests were performed, and mechanical pain threshold (MWT) and thermal pain threshold (TWL) were measured. The expressions of α-smooth muscle actin (αSMA), ETAR (endothelin A receptor), ETB (End of Transmission Block), P-Phosphatidylinositol 3-kinase (PI3K), and P-Protein kinase B (Akt) were detected by real-time fluorescence quantitative PCR and western blot. Results. In the BCP group, bone structure was severely damaged, local tissue swelling was obvious, bone trabecula was missing, and bone cortex was discontinuous. The optical density of Glial fibrillary acidic protein (GFAP) and CD11b immunoreactive signal in BCP group was significantly increased, and most of the ETAR of endothelin receptor was comapped with NeuN, and a small part of GFAP was comapped with CD11b, but no comapped with CD11b. The AS score of BQ-123+ electroacupuncture group was significantly lower than that of BQ-123 group and electroacupuncture group (
P
<
0.05
), whereas the MWT and TWL values were significantly higher than that of the BQ-123 group and electroacupuncture group (
P
<
0.05
). The mRNA expression of α-SMA and ETAR in BQ-123+ electroacupuncture group was lower than that in BQ-123 and electroacupuncture group, and the protein expression of P-PI3K and P-Akt in BQ-123+ electroacupuncture group was lower as well. Conclusion. BQ-123 may inhibit the activation of PI3K/Akt signal path combined with electroacupuncture to alleviate the effects of tibia cancer pain in rats.
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