Effects of enzymatic hydrolysis on the physicochemical and functional properties of egg yolk were investigated in this study. Alcalase, neutrase and flavourzyme were used to hydrolyze egg yolk. Solubility, foaming properties, emulsifying and microstructure properties of egg yolk were determined after enzymatic hydrolysis. Results showed that alcalase had better efficiency of hydrolysis than neutrase and flavourzyme. Enzymatic hydrolysis caused a marked changes in protein solubility, surface hydrophobicity, molecular weight distributions, microstructure and other functional properties. It was observed that egg yolk and its hydrolysates exhibited a relatively smooth curve over the entire pH range; egg yolk hydrolysates with high DH had higher solubility than those having lower DH. Foam capacity and stability generally increased with increasing DH although foam stability showed a decrease at 15% DH. Hydrolysates of egg yolk showed scattered and fewer aggregated particles. This study demonstrated that egg yolk hydrolysates could be an excellent emulsifying agent for food and other applications.
Intestinal barrier dysfunction and
inflammatory cytokine secretion
play crucial roles in inflammatory bowel disease (IBD). Herein, we
investigated the protective effects of Tricholoma matsutake-derived peptides SDIKHFPF and SDLKHFPF against dextran sulfate sodium-induced
colitis. Both peptides alleviated colitis signs, including diarrhea,
weight loss, bloody stools, colon shortening, and histopathological
changes, while reducing mucus destruction, goblet cell exhaustion,
and intestinal permeability. SDIKHFPF and SDLKHFPF protected the barrier
function by promoting the expression of tight junction (TJ) zonula
occludens-1 and occludin within the colon, as well as attenuating
colonic inflammation through myeloperoxidase and pro-inflammatory
cytokine suppression. Western blotting indicated that the peptides
suppressed myosin light chain kinase (MLCK) and nuclear factor kappa
B (NF-κB) levels, inhibiting MLC phosphorylation. SDLKHFPF was
more potent than SDIKHFPF. These findings suggest that peptide SDLKHFPF
mitigates colitis by regulating TJ protein expression and pro-inflammatory
cytokine production via NF-κB/MLCK/p-MLC signaling, improving
the barrier function.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.