Potential Mechanisms Mediating the Protective Effects ofTricholoma matsutake-Derived Peptides in Mitigating DSS-Induced Colitis

Abstract

Intestinal barrier dysfunction and inflammatory cytokine secretion play crucial roles in inflammatory bowel disease (IBD). Herein, we investigated the protective effects of Tricholoma matsutake-derived peptides SDIKHFPF and SDLKHFPF against dextran sulfate sodium-induced colitis. Both peptides alleviated colitis signs, including diarrhea, weight loss, bloody stools, colon shortening, and histopathological changes, while reducing mucus destruction, goblet cell exhaustion, and intestinal permeability. SDIKHFPF and SDLKHFPF protected the barrier function by promoting the expression of tight junction (TJ) zonula occludens-1 and occludin within the colon, as well as attenuating colonic inflammation through myeloperoxidase and pro-inflammatory cytokine suppression. Western blotting indicated that the peptides suppressed myosin light chain kinase (MLCK) and nuclear factor kappa B (NF-κB) levels, inhibiting MLC phosphorylation. SDLKHFPF was more potent than SDIKHFPF. These findings suggest that peptide SDLKHFPF mitigates colitis by regulating TJ protein expression and pro-inflammatory cytokine production via NF-κB/MLCK/p-MLC signaling, improving the barrier function.

Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury

“…These multifaceted effects confirmed the anti-inflammatory effect of T. matsutake peptides, which is consistent with the results of a previous report . Collectively, T.…”

“…DLPU2021036). After a week of acclimatization, 50 mice were equally and randomly assigned to five groups: control group treated with sterile water, gastric injury model group treated with ethanol, lansoprazole (30 mg/kg body weight [BW])-treated positive control group, and peptides SDLKHFPF (50 mg/kg BW) and SDIKHFPF (50 mg/kg BW)-treated groups. , T. matsutake peptides SDLKHFPF and SDIKHFPF were orally administered on days 1–14 as described by Wang et al ., and lansoprazole were orally administered on days 8–14; the mice were fasted for 24 h before the last administration (Figure A).…”

“…8 Furthermore, we previously identified two peptides in T. matsutake, Ser-Asp-Leu-Lys-His-Phe-Pro-Phe (SDLKHFPF) and Ser-Asp-Ile-Lys-His-Phe-Pro-Phe (SDIKHFPF), and confirmed that they can regulate the expression of tight junction proteins and the release of inflammatory factors, thereby improving the barrier function and thus ameliorating colitis. 9 Moreover, various food-derived bioactive peptides have been shown to prevent ethanol-induced gastric injury by modulating mucosal immune responses and reducing oxidative stress and inflammation. 10,11 We hypothesized that T. matsutake peptides might be effective in protecting the stomach mucosa from ethanol-triggered stomach damage.…”

l-Ergothioneine Exhibits Protective Effects against Dextran Sulfate Sodium-Induced Colitis in Mice

“… 15 Moreover, a previous study has demonstrated that the increase in MPO activity in colon tissues was generally accompanied by an increased inflammation level, and therefore as a biomarker of inflammation in mice with DSS-induced colitis. 10 Our research suggested both the EGT-4 μg group and the EGT-40 μg group significantly prevented the DSS-induced activation of MPO activity in colon tissues ( Figure 5 A), which confirmed the previous observation that EGT inhibited MPO activity in vitro. ( 15 )…”

“… 5 At present, the current effective treatment strategies for UC patients are surgery and medical therapy, which usually induce a series of complex side effects. In recent years, food-derived functional ingredients as the adjuvant treatment for UC have been gradually investigated and it is shown that dietary polysaccharides, 6 , 7 conjugated linoleic acid, 8 phenolic compounds, 9 and peptides 10 , 11 can relieve colitis by inhibiting inflammatory reactions.…”