There has been a great deal of interests in the estimation of grassland biophysical parameters such as percentage of vegetation cover (PVC), aboveground biomass, and leaf-area index with remote sensing data at the canopy scale. In this paper, the percentage of vegetation cover was estimated from vegetation indices using Moderate Resolution Imaging Spectroradiometer (MODIS) data and red-edge parameters through the first derivative spectrum from in situ hypserspectral reflectance data. Hyperspectral reflectance measurements were made on grasslands in Inner Mongolia, China, using an Analytical Spectral Devices spectroradiometer. Vegetation indices such as the difference, simple ratio, normalized difference, renormalized difference, soil-adjusted and modified soil-adjusted vegetation indices (DVI, RVI, NDVI, RDVI, SAVI L = 0.5 and MSAVI 2 ) were calculated from the hyperspectral reflectance of various vegetation covers. The percentage of vegetation cover was estimated using an unsupervised spectral-contextual classifier automatically. Relationships between percentage of vegetation cover and various vegetation indices and red-edge parameters were compared using a linear and second-order polynomial regression. Our analysis indicated that MSAVI 2 and RVI yielded more accurate estimations for a wide range of vegetation cover than other vegetation indices and red-edge parameters for the linear and second-order polynomial regression, respectively. Key words: grassland; hypserspectral remote sensing; percentage of vegetation cover; red-edge parameter; vegetation index. Liu ZY, Huang JF, Wu XH, Dong YP (2007). Comparison of vegetation indices and red-edge parameters for estimating grassland cover from canopy reflectance data.Estimation of vegetation cover is very important for assessing livestock-carrying capacity of grasslands and monitoring the extent of desertification in arid and semi-arid areas. Green vegetation cover is also an important factor in soil erosion control (Purevdorj et al.1998;Fan et al. 2002), and, therefore, a sensitive indicator of land degradation and desertification. Furthermore, quantitative information on the green vegetation cover is required in global carbon cycle studies and climate change research at the global and local scale (Qi et al.1994;Boles et al. 2004).Generally, there are two types of approaches to quantifying percentages of vegetation cover: the traditional ground-based observation versus the remote sensing approach. The former includes visual estimation, instrumental measurements and destructive sampling, which have exerted a very important value in studies of impact of climate change and plant community ecology. However, the ground-based observations are costly and time-consuming, and are difficult to execute in remotely