As one of the simplest reactive carbonyl species, formaldehyde is implicated in nervous system diseases and cancer. Organelles play crucial roles in various physiological processes in living cells. Accordingly, the detection of endogenous formaldehyde at the subcellular level is of high interest. We herein describe the development of the first organelle-targeted fluorescent formaldehyde probe (Na-FA-Lyso). The new probe exhibits favorable features including a large fluorescence enhancement (about 350-fold) and a fast response to formaldehyde. Significantly, the novel probe Na-FA-Lyso was employed to visualize the endogenous formaldehyde in the lysosomes in living cells for the first time.

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We have developed the first example of a fluorescence-enhanced and lysosome-targeted Cu 2+ probe (Lys-Cu) with unique dual-channel emissions. The new synthesized fluorescent probe Lys-Cu which contains two recognition sites with different sensoring mechanism to Cu 2+ , displays fluorescence-enhanced dual-channel emissions with fluorescence response to Cu 2+ under lysosome pH environment. Fluorescence imaging shows that Lys-Cu is membrane-permeable and suitable for visualization of Cu 2+ in lysosomes of living cells with dual-channel imaging.

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