A dual-emission fluorescence-enhanced probe for imaging copper(ii) ions in lysosomes

Abstract

We have developed the first example of a fluorescence-enhanced and lysosome-targeted Cu 2+ probe (Lys-Cu) with unique dual-channel emissions. The new synthesized fluorescent probe Lys-Cu which contains two recognition sites with different sensoring mechanism to Cu 2+ , displays fluorescence-enhanced dual-channel emissions with fluorescence response to Cu 2+ under lysosome pH environment. Fluorescence imaging shows that Lys-Cu is membrane-permeable and suitable for visualization of Cu 2+ in lysosomes of living cells with dual-channel imaging.

Highly sensitive and fast responsive ratiometric fluorescent probe for Cu2+ based on a naphthalimide-rhodamine dyad and its application in living cell imaging

“…After then, many Cu 2+ probes based on fluorescence intensity were synthesized [4,[9][10][11][12][13][14][15][16]. Although turn-on probes are more sensitive, a major limitation is that variations in the sample environment (pH, polarity, temperature, and so forth) might influence the fluorescence intensity measurements, due to the lack of background signal [17].…”

Discovery of a druggable copper-signaling pathway that drives cell plasticity and inflammation

“…Next, we confirmed CD44-dependent copper endocytosis using a fluorescent probe designed to detect copper in lysosomes. Specifically, we used Lys-Cu, whose fluorescence selectively increases upon copper(II) binding 19 . Fluorescence microscopy revealed that a fluorescently labeled HA colocalized with this probe in aMDM ( Fig.…”

A highly selective fluorescent chemosensor for Cu2+ based on a diarylethene with a 2,1,3‐benzoxadiazole unit

“…This observation was possibly due to the coordination between Cu 2+ and the 2,1,3‐benzoxadiazole N atom of 1O . Therefore, the formation of the complex 1O′ was proposed as shown in Figure according to the reported method …”