No abstract

The combination of oxfendazole and oxyclozanide is used to provide activity against fluke and gastrointestinal nematodes. This study aimed to determine both the pharmacokinetics of oxfendazole (7.5 mg/kg) and oxyclozanide (15 mg/kg) tablet formulation administered orally to sheep and whether there is a pharmacokinetic interaction between these two drugs. The study was conducted in a three-period, crossover pharmacokinetic design and on six healthy Awassi sheep 1-3 years of age.The plasma concentrations of oxfendazole and its metabolites (fenbendazole and fenbendazole sulphone) and oxyclozanide were determined by high-performance liquid chromatography using an ultraviolet detector. Compounds recovered in plasma when oxfendazole was administered alone or combined with oxyclozanide were oxfendazole, fenbendazole sulphone, and fenbendazole, respectively. When oxfendazole was administered alone and co-administered with oxyclozanide, the AUC FBZ / AUC OFZ was 0.26 and 0.23, respectively, and the AUC FBZSO2 /AUC OFZ was 0.35 and 0.32, respectively. The volume of distribution (Vz/F) of oxfendazole was large in both groups. Oxyclozanide did not change the plasma disposition of oxfendazole.When the oxyclozanide tablet formulation was administered alone, the elimination half-life (21.35 h) and the Vz/F (940.17 ml/kg) were long and large, respectively. The area under the curve (AUC) and the maximum plasma concentration of oxyclozanide were significantly larger and higher, respectively, in the oxyclozanide plus oxfendazole group (1146.61 h × μg/ml and 29.80 μg/ml) compared with the oxyclozanide group (491.44 h × μg/ml and 14.24 μg/ml) while a significant decrease in apparent Vz/F (940.17 vs 379.14 ml/kg) and total clearance (30.52 vs 13.08 ml/h/kg) was detected.In conclusion, co-administration with oxfendazole causing an increase in the plasma profile of oxyclozanide may increase the antiparasitic activity of oxyclozanide.

This study aimed to determine the effects of dexamethasone and minocycline alone and combined treatment with N-acetylcysteine (NAC) and vitamin E on serum coenzyme Q10 (CoQ10) and matrix metalloproteinase-9 (MMP-9) levels in rats administered aflatoxin B1 (AFB1).The study was carried out on 66 male Wistar rats. Following the intraperitoneal (IP) administration of AFB1 at dose of 2 mg/kg, minocycline (45 and 90 mg/kg, IP) and dexamethasone (5 and 20 mg/kg, IP) were administered alone and combined with NAC (200 mg/kg, IP) and vitamin E (600 mg/kg, IP). CoQ10 and MMP-9 levels were analyzed using the HPLC-UV method and a commercial kit by ELISA, respectively. AFB1 increased MMP-9 level and decreased CoQ10 level compared to the control group. After dexamethasone and minocycline administration, there is no increase in CoQ10 level, which is caused by AFB1. However, dexamethasone and minocycline combined with NAC+vitamin E caused significant increases in CoQ10 levels. Dexamethasone and minocycline alone and combined with NAC+vitamin E decreased MMP-9 levels compared to the single AFB1 treated group. The use of MMPs inhibitors and oxidative stress-reducing agents is anticipated to be beneficial in the poisoning with AFB1.

No abstract

Mevcut araştırmada sağlıklı koyun ve keçilere oral yolla oksfendazol, oksiklozanid ve oksfendazol-oksiklozanid kombinasyon uygulamasının, farklı zamanlarda biyokimyasal ve hematolojik parametreler üzerine etkisinin belirlenmesi amaçlandı. Çalışma çapraz dizaynda 6 adet sağlıklı dişi, 1-3 yaştaki İvesi koyun ve Alpin keçi üzerinde gerçekleştirildi. Koyun ve keçilere oral yolla 7.5 mg/kg oksfendazol, 15 mg/kg oksiklozanid ve oksfendazol-oksiklozanid (7.5 mg/kg-15 mg/kg) kombinasyonu uygulandı. Kan örnekleri ilaç uygulamalarından önce (0.saat, kontrol) ve sonraki 8., 24., 72. saat ile 7. günde alındı. Biyokimyasal parametrelerden albümin, alkalen fosfataz, alanin aminotransferaz, aspartat aminotransferaz, kolesterol, trigliserid, total protein, kan üre nitrojen, kreatinin değerleri otoanalizatör cihazında belirlendi. Hematolojik parametrelerden alyuvar sayısı, hemoglobin, hematokrit, ortalama korpüsküler hacim, ortalama korpüsküler hemoglobin, ortalama korpüsküler hemoglobin konsantrasyonu, akyuvar sayısı, lenfosit, monosit, % granülosit, % lenfosit, % monosit ve % eritrosit dağılım genişliği değerleri kan hücresi sayım cihazında ölçüldü. İlaç grupları ve zamana bağlı olarak hematolojik ve serum biyokimya parametrelerinde anlamlı bir fark bulunamadı. Bu sonuçlar koyun ve keçilere oral yolla, 7.5 mg/kg oksfendazol ve 15 mg/kg oksiklozanid tek veya kombine olarak uygulandığında hematolojik ve biyokimyasal parametrelerin fizyolojik sınırlarda olduğu ve klinik olarak anlamlı bir etkisinin olmadığını göstermektedir.