The tea green leafhopper Empoasca onukii Matsuda (Hemiptera: Cicadellidae), the orange spiny whitefly, Aleurocanthus spiniferus (Quaintanca) (Hemiptera: Aleyrodidae), and the green plant bugs Apolygus lucorum Meyer-Dür (Hemiptera: Miridae) are the important piercing–sucking herbivores in tea trees Camellia sinensis (L.) O. Kuntze (Theaceae). The goal of this study was to evaluate the laboratory toxicities and field control efficacies of botanical insecticides including matrine, azadirachtin, veratrine, and pyrethrin to three tea pests. Via leaf-dip bioassay, toxicity tests with botanical insecticides indicated that there were significant differences between the LC50 values for botanical insecticides within the same insect species. Matrine had the highest toxicity to E. onukii, A. spiniferus, and A. lucorum with the LC50 values of 2.35, 13.10, and 44.88 mg/liter, respectively. Field tests showed that, among four botanical insecticides, matrine at dose of 9 g a.i. ha−1 can significantly reduce the numbers of E. onukii and A. spiniferus and the infestation of A. lucorum on the tea plants. Furthermore, botanical insecticides matrine and azadirachtin had no obvious influence on the coccinellids, spiders, and parasitoids densities in tea plantations. The results of this study indicated that use of botanical insecticides, such as matrine, has the potential to manipulate the population of E. onukii, A. spiniferus, and A. lucorum and will be an effective and environmentally compatible strategy for the control of tea pests.
Objective
This study aimed to explore the mRNA and protein expression of SLC3A2 in laryngeal carcinoma cells and tissues, and functional regulatory mechanism of SLC3A2 in cell ferroptosis of laryngeal carcinoma.
Methods
We chose the key gene-SLC3A2 of DEGs from TCGA by bioinformatics analysis, and then we constructed stable knockdown of SLC3A2 in laryngeal carcinoma cells. MTT assay and clonogenic assay were used to determine cell viability and cell growth, respectively. The mRNA and protein expression were determined by RT-qPCR and western blotting, respectively. Xenograft tumor model was used to determine the role of SLC3A2 in tumor growth.
Results
The results of limma analysis recovered that 92 genes were involved in both upregulated DEGs and high risk of poor prognosis, whereas 36 genes were involved in both downregulated DEGs and low risk of poor prognosis. Pathway enrichment analysis indicated that mTOR signaling pathway and ferroptosis exerted a role in regulating these intersection genes. Moreover, SLC3A2 is a key gene in ferroptosis in laryngeal carcinoma. SLC3A2 is highly expressed in laryngeal carcinoma tissues and cells. Patients with high SLC3A2 expression exerted poor survival. SLC3A2 deficiency inhibited cell proliferation and foci formation. Furthermore, knockdown of SLC3A2 expression induced the efficacy of ferroptosis and suppressed ferroptosis related proteins expression. Mechanically, SLC3A2 deficiency facilitated ferroptosis through upregulating the expression of mTOR and P70S6K, whereas inhibited p-mTOR and p-P70S6K expression in laryngeal carcinoma cells. SLC3A2 deficiency inhibited tumorigenesis in nude mice.
Conclusion
Our study suggests that SLC3A2 negatively regulates ferroptosis through mTOR pathway in laryngeal carcinoma.
AZ31/Mg‐Y/AZ31 composites are fabricated by hot rolling bonding. To identify the formation mechanism of the microstructure in the interface adjacent region, additional hot rolling or/and diffusion annealing are also conducted on the base of the initial AZ31/Mg‐Y/AZ31 composite. Scanning electron microscopy, energy dispersive spectroscopy, and electron backscatter diffraction are used to characterize the microstructures and chemical compositional distributions. Vicker's microhardness tests are conducted to determine the local mechanical property. It is found that in the Mg–Y layer of the AZ31/Mg‐Y/AZ31 composite, the grain size in the interface‐adjacent region is much finer than that in the central part. Much finer Al2Y particles compared with those in the traditional Mg–Al–Y alloys are formed in the interface‐adjacent region. The formation of the fine grain in the interface‐adjacent region may be mainly related to the Al2Y particles rather than the accommodation deformation between layers. Both the grain refinement and the formation of Al2Y particles contribute to strengthening of the Mg–Y layer. The results in this work may provide a novel method to prepare fine and dispersed high‐melting‐point particles in Mg alloys, thus leading to strong and high‐thermal‐stability Mg alloys.
The laminated metal composites (LMCs) of dissimilar metals (aluminium alloys: AA1100/AA7075) were fabricated using the accumulative roll bonding technique in conjunction with cold rolling. The LMCs of ultrafine grained AA1100 and nanostructured precipitates of AA7075 achieved metallurgical bonding. The microstructure of the bonding interfaces and constituent metals was investigated using scanning electron microscopy and transmission electron microscopy for the LMCs with different layers. The deformation incompatibility and shear actions were analyzed using the microanalysis of dissimilar bonding interfaces. The mechanism of grain refinement of LMCs was investigated and described based on the microstructure characterization. The mechanical properties, strengthening mechanism, and fracture mechanism of LMCs were also investigated. The research results showed that the strengthening mechanism of LMCs is the recombination action of grain refinement, dislocation, and laminated interfacial strengthening. The coordinated deformation of dissimilar metals and the layer thickness are important in improving the mechanical properties of LMCs consisting of dissimilar metals.
The bonding interface plays an important role in the mechanical properties of laminated metal composites (LMCs). Compared with a straight interface, larger bonding area is achieved by a wavy interface, which provides higher debonding resistance for a given bonding strength. Herein, Al/Ti/Al LMCs with straight and wavy bonding interfaces are fabricated using Ti strips with initial straight and wavy profiles. The mechanical properties are investigated with in-plane uniaxial tension tests. Microstructures in the region of the interface before and after tension are characterized by scanning electron microscopy and electron backscatter diffraction. Finite element simulations of the rolling-bonding process and tension are conducted to investigate the effect of the wavy profile on the fabrication and mechanical properties of Al/Ti/Al LMCs. Compared with an initial straight profile, Al and wavy Ti strips are successfully bonded at a lower rolling reduction because of the larger local strain and higher local contact stress. Wavy interfaces between the Al and Ti layers are formed. Similar strength and ductility are obtained for Al/Ti/Al LMCs with straight and wavy interfaces when a proper rolling reduction and annealing are applied.
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