BaCKgRoUND aND aIMS: Early detection of perihilar cholangiocarcinoma (CCA) among patients with primary sclerosing cholangitis (PSC) is important to identify more people eligible for curative therapy. While many recommend CCA screening, there are divergent opinions and limited data regarding the use of ultrasound or magnetic resonance imaging (MRI) for early CCA detection, and it is unknown whether there is benefit in testing asymptomatic individuals. Our aims were to assess the diagnostic performances and prognostic implications of ultrasound and MRI-based CCA detection. appRoaCH aND ReSUltS: This is a multicenter review of 266 adults with PSC (CCA, n = 120) who underwent both an ultrasound and MRI within 3 months. Images were re-examined by radiologists who were blinded to the clinical information. Respectively, MRI had a higher area under the curve compared with ultrasound for CCA detection: 0.87 versus 0.70 for the entire cohort; 0.81 versus 0.59 for asymptomatic individuals; and 0.88 versus 0.71 for those listed for CCA transplant protocol. The absence of symptoms at CCA diagnosis was associated with improved 5-year outcomes including overall survival (82% vs. 46%, log-rank P < 0.01) and recurrence-free survival following liver transplant (89% vs. 65%, log-rank P = 0.04). Among those with asymptomatic CCA, MRI detection (compared with ultrasound) was associated with reduction in both mortality (hazard ratio, 0.10; 95% confidence interval, 0.01-0.96) and CCA progression after transplant listing (hazard ratio, 0.10; 95% confidence interval, 0.01-0.90). These benefits continued among patients who had annual monitoring and PSC for more than 1 year before CCA was diagnosed.CoNClUSIoNS: MRI is superior to ultrasound for the detection of early-stage CCA in patients with PSC. Identification of CCA before the onset of symptoms with MRI is associated with improved outcomes. (Hepatology 2021;73:1868-1881). C holangiocarcinoma (CCA) is prevalent among those with primary sclerosing cholangitis (PSC), and early detection is important. Indeed, 10%-15% of patients with PSC may develop CCA, typically the perihilar subtype. However, early-stage perihilar CCA is challenging to diagnose through noninvasive imaging, because a discrete mass can be absent, and distinguishing benign from malignant strictures is difficult. (1,2) CCA is the leading cause of death in this patient population. (3) In part, this is due to the delayed detection of CCA until advanced incurable stages. Neoadjuvant chemotherapy and radiation followed by liver transplantation
Biodegradation of phenol with pure culture of Pseudomonas putida was investigated. P. putida (PTCC 1694) was grown in facultative anaerobic condition at 27 degrees C and media pH value of 7. The effect of initial phenol concentration on the biodegradation rate was studied. The initial concentrations of phenol varied from 300 to 1000 mg/l. Experiments were performed for the duration of seven days while daily samples were withdrawn. The initial rate of biodegradation of phenol increased with initial concentration of 300-500 mg/l. Further increase in phenol concentration resulted in a slight decrease in the rate of biodegradation due to phenol inhibition. It was observed that by increasing the concentration of phenol, the lag phase was prolonged. Phenol is known to be an inhibitory substrate, thus Monod, Haldane and logistic kinetic models were applied to evaluate the growth kinetic parameters. The Monod model was unable to present the growth parameters over the defined concentration range. However, Haldane and logistic models perfectly fitted with the experimental data. The yield coefficients for the growth on phenol at concentrations of 300, 500, 700 and 1000 mg/l were 0.177, 0.062, 0.035 and 0.012 mg/mg, respectively.
Biodegradation of phenol with mixed consortia of bacterial was studied in an anaerobic batch reactor. The culture was acclimatized for a period of two months to grow in mineral salt medium contained phenol as the sole carbon source. Minimum and maximum concentrations of 50 and 1000 mg/l of phenol in the medium were used for the purpose of culture adaptation. The goals of these experiments were to determine the kinetics of biodegradation by determination of biomass growth rates and phenol concentrations with respect to incubation time. Substrate inhibition kinetics and specific growth rate were defined while data were fitted in Haldane model. The specific growth rate, half-saturation and inhibition coefficients for Haldane model were 0.067 hG , 25.32 and 200 mg/l, respectively. The biokinetic parameters were used to predict the biodegradation 1 profile, which was quite consistent with the obtained experimental data. The results were useful for the estimation of relations between growth rate and substrate utilization, which may be used to evaluate mass balance for a wastewater treatment plant contained phenol compound.
Effect of increase in phenol concentration on performance of anaerobic baffled reactor in terms of chemical oxygen demand (COD), phenol and COD removal efficiency and biogas productions was investigated. Furthermore, stability of the reactor with respect to the chemical shock load was monitored. The anaerobic baffled reactor was continuously operated with synthetic wastewater. The obtained results showed that adopted sludge improved the reactor performance. The start-up strategy used for this process has achieved to the targeted goals while the active microbial population was retained. For the initial stage of operation, there was no phenol present in the system. Then, the phenol concentrations were gradually and stepwise increased from 10 to 800 mg/L at hydraulic retention time (HRT) of 6 days. At loading rate of 0.5 g COD/L/day, maximum phenol removal efficiency of 96% was achieved. COD removal efficiency was quite high in the first compartment of baffled reactor, while the efficiency and the COD removal gradually dropped in the second and third compartments. In the bioreactor, low value of pH in the first compartment was due to acid generated by the acidogenic bacteria.
Biodegradation of phenol in the presence of glucose as a supplementary substrate was investigated with mixed microbial consortium isolated from waste effluent of coke-steel factory. Batch experiments were carried out at room temperature and pH value of 7. Initial phenol and glucose concentrations were in the range of 25-1000 mg/l and 500-3000 mg/l, respectively. In a dual substrates system the concentration of supplementary source (glucose) was kept constant. It was obvious to find out that glucose as a simple carbon source was initially utilized in the presence of phenol. The rate of phenol degradation started once glucose concentration was significantly depleted. Phenol was known to be an inhibitory substrate, thus Haldane/Andrews kinetic model was applied to evaluate the growth kinetic parameters. The kinetic parameters, mm, Ks and Ki were 0.01 1/h, 27.04 and 127.55 mg/l, respectively. The specific growth rate of the culture in dual substrates system was expressed by SKIP model (R2> 0.951). The values of interaction parameters showed uncompetitive partially inhibition at high phenol concentration.
Effects of organic loading rate and hydraulic retention time on treatment of phenolic wastewater in an anaerobic immobilized fluidized bed reactor, Journal of Environmental Engineering and Landscape Management, 22:1,[40][41][42][43][44][45][46][47][48][49] To link to this article: http://dx.Abstract. Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation. Chemical analyses revealed significant decreases in TNT concentrations, including reduction of some of the TNT to its amino derivates during the 28-day tests. The combination of bioaugmentation-biostimulation approach coupled with rye cultivation had the most profound effect on TNT degradation. Although plants enhanced the total microbial community abundance, blue fenugreek cultivation did not significantly affect the TNT degradation rate. The results from molecular analyses suggested the survival and elevation of the introduced bacterial strains throughout the experiment. Reference to this paper should be made as follows: Nõ lvak, H.; Truu, J.; Limane, B.; Truu, M.; Cepurnieks, G.; Bartkevičs, V.; Juhanson, J.; Muter, O. 2013. Microbial community changes in TNT spiked soil bioremediation trial using biostimulation, phytoremediation and bioaugmentation, Journal of Environmental Engineering and Landscape Management 21(3): 153Á162. http://dx.Abstract. Treatability of phenolic wastewater in an anaerobic immobilized fluidized bed reactor (AIFBR) in consequence of stepwise increment in phenolic load as well as decrease in hydraulic retention time (HRT) was investigated. The experimental data indicated that high degradation efficiencies of phenol and COD in the bioreactor at low HRTs and high organic loading rates were obtained. At constant HRT of 16 h with increase in influent phenol concentration from 98 to 630 mg/l, the average phenol and COD removals were 96 and 88%, respectively. However, further increase in phenol concentration in the feed stream to 995 mg/l resulted in decrease in phenol and COD removal efficiencies to 84 and 79%, respectively. For influent phenol concentration of 995 mg/l, the biogas production rate of 4.55 l/l.d was obtained. As HRT decreased from 3 to 0.15 day, the system showed high stability; influent phenol and COD were removed and reached to average values of 17 and 173 mg/l correspond to the removal efficiencies of about 97 and 90.5%, respectively. The bioreactor experienced a failure with further decrease in HRT to 0.1 day. Biogas production was gradually decreased from 7.04 l/l.d at HRT of 3 days to 2.23 l/l.d at HRT of 0.1 days. The value of the ratio of volatile fatty acids to total alkalini...
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