BackgroundPMS2 expression loss was reported in a variety of human. However, its importance has not been fully understood in cervical carcinoma. The aim of this study was to determine the expression of PMS2 in cervical carcinoma and evaluate the significance of mismatch repair gene PMS2 regulated by glycogen synthase kinase 3β (GSK-3β) in chemosensitivity.MethodsWe examined PMS2 and phosphorylated GSK-3β(s9) expression in cervical carcinoma tissues using immunohistochemical staining. Furthermore, we detected PMS2 expression in HeLa cells and evaluate the interaction with GSK-3β after transfection with GSK-3β by small interference RNA (siRNA), co-immunoprecipitation and immunoblotting. We also evaluated the effect of PMS2 transfection on HeLa cells' chemosensitivity to cisplatin treatment.ResultsWe found significant downregulation of PMS2 in cervical carcinoma, which was negatively associated with phosphorylated GSK-3β (s9). Furthermore, we demonstrated GSK-3β transfection was able to interact with PMS2 and enhance PMS2 production in HeLa cells, and increased PMS2 production was responsible for enhanced chemosensitivity.ConclusionsOur results provide the evidence that stabilization of PMS2 production by GSK-3β was important to improve chemosensitization, indicating the significance of GSK-3β-related PMS2 downregulation in the development of cervical carcinoma and in developing a potential strategy for chemotherapy.
With the aim to obtain the high cavitation-erosion-resistance coating for the surface safeguard of fluid machinery, two kinds of hard coatings (WC-Cr-Co and Cr3C2-NiCr) were prepared on 1Cr18Ni9Ti substrate by high velocity oxy-fuel spraying (HVOF), which microstructure and performance were investigated respectively by optical microscope, X-ray diffraction, Vickers hardness, scanning electron microscope (SEM), and vibratory cavitation apparatus. The results indicated that these two coatings had the higher hardness and the finer structure than ZG06Cr13Ni5Mo, which is used actual in water conservancy. The results of cavitation erosion continued for 39 hours presented that the mass loss of WC-Cr-Co and Cr3C2 -NiCr coatings increased ceaselessly with time, and the cavitation erosion speeds varied with time. Compared with ZG06Cr13Ni5Mo, their cavitation erosion resistance performances were better due to their higher hardness and finer structure, and WC-Cr-Co coating was much better than Cr3C2-NiCr coating. Obviously, the higher hardness and finer structure could strengthen the cavitation erosion resistance of coating materials.
Effect of Cr element on microstructure of Al-3.2Mg alloys was investigated using optical microscopy, scanning electron microscopy (SEM) and EDS. Moreover, the tensile and hardness test were carried out. The results show that a minor additive of Cr can refine effectively the secondary dendrite arm spacing and decrease the number of coarse interdendritic structures. In the whole series of the alloys, the secondary dendrite arm spacing of Al–3.2Mg-0.1Cr alloy was the smallest, and the spacing presented an increasing trend with the Cr content. With high levels of Cr, the intermetallic compounds are formed by Cr with Al, Mg and Fe, the shape of the second phase particles were transformed from bulky bone shape to fine bone shape and long striped. The strength and the hardness of Al–3.2Mg alloys were greatly improved when the additions of Cr were 0.05% and 0.1%, but the ductility of the alloys remained at a low level by adding 0.05%Cr. Therefore, combining with the comprehensive properties and the microstructure, the best addition of Cr in the alloy was 0.10% in the whole series.
To ensure its operational security, it is important to study the vibration state of transmission line. The equivalent viscous damping of aero-vibration for a large-span overhead conductor is obligated to be determined. In this paper, the damping characteristics of conductor by AACSR-400 steel core aluminum alloy wire are studied by use of energy balance principle. Based on the Diana wind energy curve and equivalent viscous damping, the relationship between frequency and damping ratio is conducted and discussed. Furthermore, a numerical simulation by ANSYS is performed to verify the validity of the equivalent damping coefficient and equivalent damping ratio with the same material parameters. Study shows that the result of finite element method by ANSYS is consistent well with that by energy balance principle.
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