Diabetic foot infection is one of the terrible complications of diabetes leading to morbidity and mortality. This study evaluated the resistance and sensitivity of isolated aerobic bacterial frequencies that were isolated from patients with diabetic food for the commonly used antibiotics to assist in identifying empirical therapy.This study aimed to determine the antibiotic sensitivity and resistance pattern to detect aerobic bacterial pathogens present in diabetes pus against 13 commonly used standard antibiotics: erythromycin, oxacillin, chloramphenicol, ciprofloxacin, vancomycin, methycillin, amoxicillin, cephoprazin, and biphenylpropionone.Forty patients with a diabetic foot infection, including 28 males and 12 females from Al Najaf center for diabetes and endocrine in Al-Sadir medical city, were clinically sampled for 5 months. All swabs from these 40 patients showed a positive culture and a total of 98 isolates were isolated.Standard aerobic microbiological techniques were used to process clinical specimens. Also the Kirby-Bauer disc diffusion method was used to study the antimicrobial susceptibility pattern.The Infections are generally due to Gram-positive bacteria. The most isolated Gram-positive bacteria was Staphylococcus aureus bacteria and streptococcus spp. followed by Staphylococcus aureus and Bacillus spp. (49%, 34%, 13%and 3%), respectively. While Proteus spp. and Pseudomonas spp. the most isolated Gram-negative bacteria followed by Escherichia coli and Klebsiella spp. and Enterobacter spp. and Acenetobacter baumannii (32%, 27%, 19%, 11%, 5% and 5%), respectively.
In this study, 20 swabs were collected from three hospitals in the holy city of Karbala (Al Hussein General Hospital, Al Abbas Private Hospital and Maternity Hospital). Nutrient agar was grown on solid feed medium and incubated at 37 ° C for 24-48 hours for isolation and diagnosis. Bacterial isolates obtained underwent biochemical and bacteriological tests for diagnosis. I prepared different doses of isolated bacteria according to the McFarland model. Then take 0.1 ml of 1/10000 IV dilution and add to Muller Hinton agar medium. The results of the preliminary diagnosis of the isolated bacteria showed that the positive bacteria of Cram stain were higher than those of Cram negative bacteria. The largest number of S.aureus bacteria was in the hospital environment from which swabs were taken and in all locations, especially bathrooms. By calculating the percentage of the total number of samples diagnosed for each hospital separately, the results showed that Hussein General Hospital had the highest contamination rate of 90%, while Al-Ahli Hospital had the lowest contamination rate of 40%. With regard to the effect of antiseptics, the concentration gave 75% Dettol the highest amount of inhibition of bacterial growth compared with other concentrations. On the other hand, the synergistic effect of both antiseptics (Dettol and chlorine) 75% -25% had an effect on inhibition of bacterial growth and its total elimination in the medium compared to other concentrations.
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