Aerobic Bacteria Isolated From Diabetics Foot Infection Locally and Antibiotics Influence

Abstract

Diabetic foot infection is one of the terrible complications of diabetes leading to morbidity and mortality. This study evaluated the resistance and sensitivity of isolated aerobic bacterial frequencies that were isolated from patients with diabetic food for the commonly used antibiotics to assist in identifying empirical therapy.This study aimed to determine the antibiotic sensitivity and resistance pattern to detect aerobic bacterial pathogens present in diabetes pus against 13 commonly used standard antibiotics: erythromycin, oxacillin, chloramphenicol, ciprofloxacin, vancomycin, methycillin, amoxicillin, cephoprazin, and biphenylpropionone.Forty patients with a diabetic foot infection, including 28 males and 12 females from Al Najaf center for diabetes and endocrine in Al-Sadir medical city, were clinically sampled for 5 months. All swabs from these 40 patients showed a positive culture and a total of 98 isolates were isolated.Standard aerobic microbiological techniques were used to process clinical specimens. Also the Kirby-Bauer disc diffusion method was used to study the antimicrobial susceptibility pattern.The Infections are generally due to Gram-positive bacteria. The most isolated Gram-positive bacteria was Staphylococcus aureus bacteria and streptococcus spp. followed by Staphylococcus aureus and Bacillus spp. (49%, 34%, 13%and 3%), respectively. While Proteus spp. and Pseudomonas spp. the most isolated Gram-negative bacteria followed by Escherichia coli and Klebsiella spp. and Enterobacter spp. and Acenetobacter baumannii (32%, 27%, 19%, 11%, 5% and 5%), respectively.

ß-Lactamase and Antibiogram in Some Gram-Negative Bacteria Isolated from Foot Ulcer Patients

“…The samples of this study showed that 80 of the cases that were taken 75 (93.75%) showed positive culture including different types of gram-negative bacteria pattern against different types of antibodies so we just isolate and detect gram negative bacteria. A total of 115 pathogens were isolated just from gram negative bacterial isolates as showed in table (1). Table (1) shows the number and types of isolates of gram-negative bacteria that were isolated from the patients with diabetic foot ulcer these types of bacteria include E. coli, Klebsiella spp., pseudomonas spp.…”

“…A total of 115 pathogens were isolated just from gram negative bacterial isolates as showed in table (1). Table (1) shows the number and types of isolates of gram-negative bacteria that were isolated from the patients with diabetic foot ulcer these types of bacteria include E. coli, Klebsiella spp., pseudomonas spp. and Proteus spp.…”

“…The development of chronic diabetes complications is associated with a large part of the burden of the disease, as well as the cost of health care as the most common complication is diabetic foot disease that diabetics fear (1). Diabetic foot is characterized by several pathological complications such as neuropathy, peripheral vascular disease, foot ulceration and infection with or without osteomyelitis, leading to the development of gangrene and even necessitation limb amputation (2).…”