Rapid identification of bacteria is critical in clinical and food safety applications. This paper describes a novel instrument and data analysis method for identifying bacteria based on the measurement of laser light scattering as the beam interacts with bacterial cells suspended in water. A description of the technology is followed by an identification performance study for a set of strains from the genus Staphylococcus (the inclusive target organisms) and a set of non-Staphylococcus strains (the exclusive organisms). Staphylococcus and non-Staphylococcus cells were grown on sheep blood agar (SBA), tryptic soy agar, brain heart infusion (BHI) agar, or Luria-Bertani (LB) agar and identified based on how cells scattered light. Bacteria from the genus Staphylococcus grown on solid media were correctly identified more than 92% of the time. To determine whether the system could also identify bacteria grown in liquid culture, six different Staphylococcus strains and six different non-Staphylococcus strains were grown in tryptic soy broth, BHI broth, or LB broth. This system accurately identified all targeted Staphylococcus samples tested, and no misidentifications occurred. A single-blind identification experiment was also performed on human clinical isolates obtained from the Upper Peninsula Health System. Ninety blind-coded clinical bacterial isolates on SBA were tested to determine whether they were from the genus Staphylococcus. All Staphylococcus were accurately identified, and no misidentifications occurred. This study demonstrated the proof of concept of a novel system that can rapidly and accurately identify bacteria from pure culture based on cellular light-scattering properties.
The NO releasing film dressings resulted in significantly lower bacterial colony counts as compared to the control dressings at all time points. Incorporating a NO compound into a dressing has an antibacterial effect lasting a minimum of seven days.
Case: We present a case of acute idiopathic four-compartment syndrome of the leg, treated by four-compartment fasciotomy, and wounds left to heal by secondary intention due to persistent edema following surgery. Conclusion:This case highlights the importance of maintaining a high level of clinical suspicion for idiopathic spontaneous compartment syndrome presentation.This case also illustrates the variability of compartment syndrome treatment and recovery. The standard treatment for compartment syndrome is fasciotomy with delayed primary wound closure, but the patient elected to heal by secondary intention. The patient's long term follow-up results showed positive outcomes.
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