Summary
The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.
The Bombyx mori sex determination gene Bmdsx is alternatively spliced in the male and female to produce the male- and female-specific proteins. In an effort to better understand the mechanism of the alternative splicing regulation of Bmdsx, we conducted a gel-shift assay followed by LC-MS/MS analysis to identify the putative proteins bound to the cis-element CE1+6 in the exon 4 of Bmdsx. A protein named as BmHrp28 which is homologous to the Drosophila Hrp48, a member of the hnRNPA/B family, was identified and expressed in Escherichia coli for testing RNA-protein binding in vitro. All of the results showed that BmHrp28 specifically bound to the CE1+6 RNA probe. BmHrp28 has two RNA recognition motifs at the N-terminal and a glycine-rich motif at the C-terminal. It might be one of the factors involved in the male-specific splicing of Bmdsx.
As a traditional processing method, brining is a preliminary, critical and even essential process for many traditional rabbit meat products in China. The aim of this work was to investigate mass transfer of rabbit meat brined in different salt concentration. Rabbit meat (Longissimus dorsi) was brined for 24 h in 5 brine solutions (5, 10, 15, 20 and 25% NaCl [w/w]). Results indicated that mass transfer and kinetics parameters were significantly affected by the brine concentration during brining. When brine concentration increased, the total and water weight changes decreased, whereas the sodium chloride weight changes increased. Higher brine concentrations resulted in a higher degree of protein denaturation and consequently gave lower process yields. Samples treated with higher brine concentrations obtained lower brining kinetic parameter values for total weight changes and water weight changes, whereas they acquired higher values for sodium chloride weight changes.
A protective Ni–Co alloy coating was successfully prepared on sintered neodymium–iron–boron (NdFeB) magnet via potentiodynamic electrodeposition in this paper. The microstructure and surface morphology analysis of Ni–Co alloy coating showed that the surface morphologies were quite compact and more uniform than that prepared by direct current (DC) electrodeposition; significantly, the chemical composition analysis indicated that the content of Co in the alloy coatings amounted to 33–34 wt-. Potentiodynamic polarisation and long term immersion test results revealed that the Ni–Co alloy coating could supply excellent corrosion protection for NdFeB substrate in 3.5 wt- NaCl solution. Overall, the Ni–Co alloy coating with higher Co content prepared by potentiodynamic electrodeposition exhibited better corrosion protection for sintered NdFeB magnet compared with that prepared by DC electrodeposition.
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