After the All-Sky Automated Survey for SuperNovae (ASAS-SN) discovered a significant brightening of the inner region of NGC 2617, we began a ∼ 70 day photometric and spectroscopic monitoring campaign from the X-ray through near-infrared (NIR) wavelengths. We report that NGC 2617 went through a dramatic outburst, during which its X-ray flux increased by over an order of magnitude followed by an increase of its optical/ultraviolet (UV) continuum flux by almost an order of magnitude. NGC 2617, classified as a Seyfert 1.8 galaxy in 2003, is now a Seyfert 1 due to the appearance of broad optical emission lines and a continuum blue bump. Such "changing look Active Galactic Nuclei (AGN)" are rare and provide us with important insights about AGN physics. Based on the Hβ line width and the radius-luminosity relation, we estimate the mass of central black hole to be (4 ± 1) × 10 7 M ⊙ . When we crosscorrelate the light curves, we find that the disk emission lags the X-rays, with the lag becoming longer as we move from the UV (2 − 3 days) to the NIR (6 − 9 days). Also, the NIR is more heavily temporally smoothed than the UV. This can largely be explained by a simple model of a thermally emitting thin disk around a black hole of the estimated mass that is illuminated by the observed, variable X-ray fluxes.

Las Cumbres Observatory Global Telescope (LCOGT) is a young organization dedicated to time-domain observations at optical and (potentially) near-IR wavelengths. To this end, LCOGT is constructing a world-wide network of telescopes, including the two 2m Faulkes telescopes, as many as 17 x 1m telescopes, and as many as 23 x 40cm telescopes. These telescopes initially will be outfitted for imaging and (excepting the 40cm telescopes) spectroscopy at wavelengths between the atmospheric UV cutoff and the roughly 1-micron limit of silicon detectors. Since the first of LCOGT's 1m telescopes are now being deployed, we lay out here LCOGT's scientific goals and the requirements that these goals place on network architecture and performance, we summarize the network's present and projected level of development, and we describe our expected schedule for completing it. In the bulk of the paper, we describe in detail the technical approaches that we have adopted to attain the desired performance. In particular, we discuss our choices for the number and location of network sites, for the number and sizes of telescopes, for the specifications of the first generation of instruments, for the software that will schedule and control the network's telescopes and reduce and archive its data, and for the structure of the scientific and educational programs for which the network will provide observations.Comment: 59 pages, 9 figures, 4 tables. AAS Latex v5.2. Accepted for publication in Pub. Astr. Soc. Pacifi

We analyze a ∆V ∼ −9 magnitude flare on the newly identified M8 dwarf SDSS J022116.84+194020.4 (hereafter SDSSJ0221) detected as part of the All-Sky Automated Survey for Supernovae (ASAS-SN). Using infrared and optical spectra, we confirm that SDSSJ0221 is a relatively nearby (d∼76 pc) M8 dwarf with strong quiescent Hα emission. Based on kinematics and the absence of features consistent with low-gravity (young) ultracool dwarfs, we place a lower limit of 200 Myr on the age of SDSSJ0221. When modeled with a simple, classical flare light-curve, this flare is consistent with a total U -band flare energy E U ∼ 10 34 erg, confirming that the most dramatic flares are not limited to warmer, more massive stars. Scaled to include a rough estimate of the emission line contribution to the V band, we estimate a blackbody filling factor of ∼10-30% during the flare peak and ∼0.5-1.6% during the flare decay phase. These filling factors correspond to flare areas that are an order of magnitude larger than those measured for most mid-M dwarf flares.

Nucleic acid amplification tests for Mycobacterium tuberculosis (MTB) detection from sputum are highly sensitive and specific with smear microscopy positive specimens, but their sensitivity with smear-negative/culture-positive specimens is much lower; therefore, these tests cannot rule out a tuberculosis diagnosis. Co-extraction of PCR inhibitors may be a cause of decreased test sensitivity. Here the design and early validation of a MTB screening assay with sample preparation and qPCR methods designed to specifically address this diagnostic gap is reported. First, human genomic DNA is identified as a significant qPCR inhibitor. To circumvent this problem, a novel, streamlined sample preparation method utilizing detergent and proteolysis to thin the sputum and DNA sequence specific MTB DNA isolation was developed. Additionally, a multiplexed qPCR assay targeting two MTB complex-specific loci: the potentially multi-copy IS6110 and the single-copy senX3-regX3, combined with the cotJC gene from Bacillus atrophaeus spores amplified as a process control was developed. The limit of detection of the test was estimated to be 20 cfu/ml which is significantly lower than the Xpert MTB/RIF assay. In a preliminary field study of 60 de-identified blinded sputa, a test sensitivity of 96% and specificity of 100% was observed when compared to the Xpert MTB/RIF assay.