Plant flavonoids are polyphenolic compounds, commonly found in vegetables, fruits and many food sources that form a significant portion of our diet. These compounds act as anticancer and anti-proliferative but after interaction with milk proteins they form complexes which become less effective than these compounds alone. Investigation has been conducted to delineate the action of some phenolic compounds of natural origin and complexes formed from interactions between phenolic compounds and milk proteins in three human tumors cell lines: Breast (MCF7), Liver (HePG2) and colon (HCT116), these interactions were studied by fourier transform infrared spectroscopy (FTIR). Phenolic compounds have positive effect on human cancer cell lines but after interaction with milk protein and forming complexes, they become less effective than phenolics and some time have negative effect and become pro-cancer; this interaction can be studied by (FTIR) to know which groups do this complex. The spectra were recorded under conditions generally applied in quantitative work. IR spectra were recorded in the region from 4000-250 cm-1 , but the bands in the region 4000-1400 cm-1 were analyzed in detail, since they are characteristic of OH groups while NH groups appeared at (3000-4000 cm −1) of various protonic species that undergo hydrogen bonding interaction. Another region of interest was the region from 1800-1400 cm-1 , characteristic of the bending vibrations of the same group. Since the bands in this region were wide and complex.

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Eco‐friendly selenium nanoparticles (SeNPs) were prepared by biological‐methods using three individual or mixed strains of lactobacilli, which were evaluated for yield, selenium content, and particle size. The prepared SeNPs were used to fortify yoghurt, and the product was evaluated for its chemical composition, rheological properties, and microbiological quality. The highest SeNPs yield was obtained using Lactobacillus casei (26.87 g/L), whereas L. acidophilus produced nanoparticles with a significantly high selenium content. The lowest particle size (131.0 nm) was found in eco‐friendly SeNPs produced using mixed strains followed by those produced using L. plantarum (146.2 nm), L. acidophilus (202.8 nm), and L. casei (271.3 nm). Adding eco‐friendly SeNPs had no notable effect on the chemical composition and texture of functional yoghurt. Eco‐friendly SeNPs powder prepared with L. plantarum had the highest viable count, whereas the lowest was obtained using the mixed culture. No cytotoxicity was observed in a tested colon cancer cell line for all prepared eco‐friendly SeNPs. Practical applications The methods used in the preparation of selenium nanoparticles using lactic acid bacteria are safe, cheap, and easy to apply. It can be applied on an industrial scale as one of the ingredients that can be used to safely support different foods. Selenium nanoparticle prepared in this way can be added in the fortification of milk, children's food, and also bakery products to meet their daily needs of the important selenium element. They can also be produced in tablets containing the daily dose of selenium and also supported by probiotic bacteria. Therefore, the method used to prepare selenium nanoparticle in this study is one of the most promising ways to be applied in different food products.

Key words:Histamine, HPLC, ELISA, Marine fish, Essential oil.Our a complete of 60 samples of fresh marine fishes represented by Sardine, Saurus, Barboni and Chrysophyres were collected at different times from various fish markets in Alexandria governorate, Egypt and examined for determination of their levels of histamine by HPLC from one side and ELISA from the other side to study the accuracy of the two techniques. Results showed that all of specimens contained detectable value of histamine but the highest unacceptable sample in sardine fish and lowest unacceptable sample in Chrysophyres fish and there is no significant differences (P<0.01) as indicated by HPLC and ELISA test for detection of histamine. Further, some trials to control the formation of such serious histamine level (mg %) were treated by using 1% essential oils (Rosemary oil, Garlic oil and Lemon oil) by the use of HPLC. The outcomes indicated that the development of histamine formation become simply decreased by the percentage of 15.2% ,47.8 and 58.2% with addition of Rosemary oil (1%) for 2 ,4 days and 6 days ,respectively; 9.9 % , 35.3% and 45.7% after addition of garlic oil(1%) for two ,four days and six days , respectively and the best reduction degree in histamine development changed into recorded through the addition of Lemon oil (1%) 2.1% , 21.5% and 29.3% for two ,four days and six days ,respectively. In conclusion, such results can propose that the essential oil treatment to the fish fillets might be helpful in reduction of histamine formation so that it will produce fish meat of excessive best and protection.

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