The analysis of scientific literature shows that the study of adrenal morphology in humans and animals is an urgent problem of biology and medicine from ancient times to the present day. In the historical aspect, we can distinguish three stages of studying the adrenal gland that differ in the directions of research. The first stage corresponds to the research of scientists of the XVI–XVIII centuries (Bartolomeo Eustachio, Andreas Vesalius, Gabriele Folloppio, Girolamo Fabrici, Andriaan Spieghel, Johann Vesling, Giulia Casseria, Caspar Bauhin, Johann Grafenberg, Caspar Bartholin, Thomas Bartholin, Giulio Casserio, Antonio Molinetti, Jean Riolan, Thomas Wharton, Giovanni Lancisi, Jakob Winslow, Antony Valsalva, Albrecht Haller, Johann Meckel, Jean Senac, Armand Cassan). Their works is devoted to the study of topography, macroscopic structure and function of the adrenal gland. Studies of morphological scientists of the second stage (late XVIII – mid XX century) correspond to the study of the structure of the adrenal gland at the microscopic level. Scientists like Albert Kelliker, Johann Ecker, Thomas Addison, Gabriel Colin, Alfred Kohn and Jay Arnold used histological and histochemical research methods. Edward Schafer, George Oliver, Vladislav Szymonowicz, John Abel, Jokiti Takmine, Welter Cannon, Edward Kendall to the study of the features of adrenal hormone secretion. Scientific works of the mid-twentieth and early twenty-first centuries can be combined in the third stage of research on the morphology of the adrenal gland. It is devoted to solving issues related to the formation of the adrenal gland in the process of filogenesis and ontogenesis, the features of the structure and function of the organ in normal and pathological conditions. A significant contribution to the study of the morphology of the adrenal gland of animals at the third stage was made by such scientists as Ziyade A. M., Dardykina O. N., Harina V. V., Atagimov M. Z., Torguj P. M., Antipin I. A., Shishkin A. P., Volkova M. V., Shevchenko L. F., Sidorova O. G., Vovchenko M. B., Salekh M. M., Ovcharenko N. D., Zaika S. V., Samatova I. M., Gorbacheva E. S., Pronin V. V., Kuznecov A. V., Pashinin N. S., Strel’nikova I. G., Barvenko A. D., Fedotov D. N., Izatulin A. V., Kvarackheliya A. G., Silkina A. V., Muhametov A. I. Among the studies of morphologists of the third period, the method of electron microscopy prevails.
Aim. To determine the difference in immune responses of the harderian gland in clinically healthy chickens and the ones with infectious bronchitis based on the content, localization and morphometric estimation of the surface markers of Т- and В-lymphocytes and to determine the differentiation index as an indicator of assessing body defenses. Methods. Histological, immunohistochemical, optical, morphometric and statistical. Results. The histological study of the harderian gland of chickens with infectious bronchitis determined the swelling and proliferation of the connective tissue as well as infiltration of secretory lobules by lymphoid cells. It was found that the immunity of chickens with infectious bronchitis, in which the harderian gland plays a relevant role, depends considerably on the differentiation index of immunocompetent cells. There was a reliable 1.77- and 1.36-fold decrease in this indicator for 40- and 90-day-old chickens, respectively, in case of nephroso-nephritic form of infectious bronchitis which demonstrated a weaker function of the defense cells of this organ. According to the cytomorphometric analysis, the number of cells, expressing CD4+, CD8+, CD20+, CD45RA+ markers in the harderian gland of sick 20-, 40-, and 90-day-old chickens with respiratory and nephroso-nephritic forms of infectious bronchitis was reliably (P < 0.05) increasing compared to the clinically healthy chickens. For instance, the number of mature В-lymphocytes increased in sick 20-day-old chickens – 2.44 times, 40-day-old chickens – 1.88 times, and 90-day-old ones – 2.62 times compared to clinically healthy chickens. Conclusions. The data were obtained about the changes in quantitative and qualitative composition of lymphocytes with surface markers CD4+, CD8+, CD20+, CD45RA+ in the harderian gland of chickens with infectious bronchitis. Our results will supplement current knowledge about the feasibility of immunohistochemical methods in the diagnostics of avian infectious bronchitis.
The article presents the data of pathomorphology of chicken organs for infectious bronchitis. The authors found that the lesions of the organs for infectious bronchitis of chickens are systemic in nature and manifests itself depending on the age of the bird and the form of the disease: in the respiratory form, the lungs are most affected in chickens of 20-day age; in the nephroso-nephritis formkidneys at 40, 90-day age; in the reproductive formovaries and ovaries at 285-day age. In this case, the respiratory form of infectious bronchitis in the lungs of chickens was observed hyperemia and edema of stroma, in pnevmocapillar and around the vessel contains a transudate. A significant number of pneumocapillars filled desquamated epithelium and lymphocytes. Some parts of the lung tissue and the tissue around the vessels are impregnated with transudate. In the lumen of parabronchus the presence of desquamated epithelium, lymphocytes, and muco-serous exudate noted. There were large areas of atelectasis, which bordered on areas of compensatory emphysema. The disease was accompanied by catarrhalhemorrhagic, croup inflammation of the bronchus. In many cases, hyperemia and hemorrhages on the mucous membrane of the trachea, often catarrhal bronchitis, were observed. For nephroso-nephritis forms of infectious bronchitis in the kidneys of chickens for infectious bronchitis, hyperemia and hemorrhages in the stroma, granular dystrophy, necrosis and desquamation of tubular epithelial cells were recorded, in the lumen of which the accumulation of protein mass was detected, as well as blood filling of the capillary plexus of the vascular glomerulus. According to the reproductive form of infectious bronchitis in the oviduct, the presence of cysts was noted, especially in the anterior part of it. The posterior part of the oviduct was predominantly atrophied. The ovary was significantly reduced and covered with yellow bodies. In the future, atresia and degenerative changes in the egg follicles occurred.
The article describes the features of the macroscopic and microscopic structure of the liver of mature clinically healthy dogs for the use of anatomical, histological and morphometric methods of research. According to the results of organometallic and cytometric studies, the absolute and relative body mass, the volume of hepatocytes, their nuclei and the nuclear-cytoplasmic ratio were determined. Thus, the absolute weight of the liver in the mature dogs is variable and to a certain extent depends on the blood flow to the organ and is 427.4 ± 21.92 g, the relative weight – 2.69 ± 0.1%. Liver of mature dogs is a compact body of flattened form with sharp lower and lateral edges, with deep cuts, dark red color. The histoarhitectonics of the liver of dogs are constructed with connective tissue and parenchyma. The stroma of the organ is formed by a capsule, on top of which is serous membrane. In the area of the gates of the liver, the connective tissue of the capsule penetrates into the middle of the organ, branching and dividing it into lobules. Then it is formed by the hepatic plates by the intraosseous sinusoid capillaries. Liver plates are specific endpoint of the secretory unit of the liver. It has been found that in the microscopic structure of the liver of the dogs, the interstitial connective tissue is poorly developed, therefore the boundaries between the liver lobules are not sufficient. The liver plates behind the microscopic structure have a radial direction from the center to the periphery. The direction closer to the periphery of the particles is less noticeable, since hepatocytes are located in two rows, between which the sinusoidal space clearly appears. Hepatocytes had an irregular, multifaceted form. Their nuclei, which were mainly in the center of the cell, had little clarity of contours of carriel and well perceived color. During microscopic examination of the liver, it is swollen that hepatocytes of the central and intermediate zones are better perceived by color than the cytoplasm of the cells of the peripheral zone of the liver. According to the analysis of our cytomorphometric studies, hepatocytes of dogs have different sizes, which vary in wide range: from small to large. Whereas the average volume of hepatocytes in mature dogs is 964.72 ± 56.003 μm³; the average volume of their nuclei is 105.13 ± 2.02 microns. The nuclear-cytoplasmic ratio of hepatocytes in dogs of this group is 0.128 ± 0.122.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.