Environmental DNA or eDNA utilizes traceable genetic materials in the environment for monitoring the presence of organisms in a given area and it is now gaining popularity as an alternative for traditional monitoring methods. Thus, the selection of genetic markers is crucial for identification of species in wildlife monitoring. This paper aims to review several DNA markers which are appropriate and reliable for detection of organisms from the environmental samples. We performed systematic literature search from SCOPUS database to review all molecular markers of eDNA. This study focuses on the importance of markers selection which can be utilized by next-generation sequencing (NGS) for biodiversity monitoring. Cytochrome C oxidase Subunit I (COI) are noted as the most widely used marker in metabarcoding research for detection of targeted species.
Preserving animal’s body or part of the animals have become an important and well-known conservation medium to ensure that the future generations are still able to enjoy the biodiversity and all the incredible species that live within it. This paper aims to present a qualitative study on perception and preference of subject matter experts (SMEs) on various curation and preservation methods that is suitable for certain animals for the development of Natural History Gallery. Interview with the SMEs were done by using semi-structured questionnaire and prior to this, permission were gained from the respondents by signing the Informed Consent Form (ICF). The findings suggest that the preservation methods of specimens ultimately depend on the purpose of specimens collected, either to be preserved as research specimens or exhibition specimens. The information gained form this study will be valuable as directive for the development of natural history gallery in Malaysia.
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