The gtjT gene and its upstream region isolated from the Streptococcus sobrinus OMZ176 chromosomal DNA were sequenced. The g(JT gene was preceded by a potential Shine-Dalgarno sequence. The g(fT gene product, glucosyltransferase (GTF), displays a typical gram-positive bacterial signal peptide sequence and both an active site peptide sequence and carboxy-terminal repeats typical of GTFs. The signal sequence is similar to those of other known GTF proteins. The putative active-site peptide sequence of this enzyme was DGIRVDAVD, which was different by one amino acid from the active-site peptide sequence derived from two different types of the S. sobyinus GTFs reported previously (G.
A glucosyltransferase (GTF) gene was cloned into Escherichia coli from serotype d Streptococcus sobrinus OMZ176. Transformed E. coli strain MI expressed water-insoluble glucan synthesizing activity. Restriction enzyme map of pGT31 extracted from MI shows that the enzyme gene exists in the 6.4-KB PstI-fragment inserted into pBR322 vector. DNA sequence analysis indicates that a single ORF (530-5,300) is located in the PstI-fragment. The putative amino-acid composition (1,590 residues) resembles those of other GTF-I enzymes obtained from serotype g S. sobrinus and serotype h Streptococcus downei. However, at the same positions of the sequence, 18 and 142 amino-acid residues are different between serotype d and g, and serotype d and h GTF-I enzymes, respectively. The differences between serotype d and h GTF-Is are distributed in N and C-terminal regions.
The purpose of this study was to evaluate the characteristics of human resources for health promotion advocacy in a rural community. A 16-item questionnaire was sent to 1000 residents of a rural Japanese town. Items concerned demographics, participation in organizational activities, awareness of volunteer activities, and willingness to participate in community-based oral health activities. Responses were measured on a 5-point scale. The response rate was 57.4%. To reveal potential factors from the distribution of responses, a principal component analysis was performed, and 4 components were extracted. The components were interpreted as "Municipality initiative," "Personal initiative," "Worth volunteering," and "Internal health locus of control (HLC)," respectively. The results of multiple regression analysis using the above components stratified by gender and age- group revealed that potential leaders in oral health activities in Japan are presently participating in social activities of their own accord and highly value volunteer activity. Target groups are senior males and middle-aged females.
The purpose of this investigation is to grasp the actual condition of malocclusion in Japanese junior high school pupils using the Dental Aesthetic Index (DAI). A total of 218 junior high school pupils aged 12-13 years were examined according to DAI. About 40% of the subjects had incisal crowding. The percentage of subjects with incisal spacing was 24%, and 71% of them had diastema of 1 mm or more. The percentage of subjects with anterior irregularity on the upper and lower arch was 39% and 33%, respectively. The percentage of subjects with anterior maxillary overjet and mandibular protrusion of 1 mm or more was 85% and 6% respectively. Dislocation by more than a half-cusp in the proximal or distal relationship in the molars was observed in 26% of all subjects. Mean +/- SD of DAI score was 25.3 +/- 7.3.
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