Different kinds of lactobacilli and Bifidobacteria fermented milk were fed to ovalbumin-specific IgE-elevated mice for 3 days, and after the final administration, changes in the ovalbumin-specific IgE values for each sample were compared to the value for non-fermented milk. Seven of the Lactobacillus-fermented milks caused a significant decrease in the serum ovalbumin-specific IgE levels. Above all, Lactobacillus acidophilus L92, Lactobacillus acidophilus CP1613, and Lactobacillus fermentum CP34 fermented milk had the most significant effects of decreasing the serum ovalbumin-specific IgE levels compared to a control group. The L. acidophilus L92 and L. fermentum CP34 cells also showed significant ovalbumin-specific IgE lowering activities. From these results, an active component seems to exist in the cells of L. acidophilus L92 and L. fermentum CP34 strains. Recovery of the radiolabeled L. acidophilus L92 and L. fermentum CP34 cells from the small intestine and the large intestine of the mouse 13 h after oral administration were higher than the recovery of any other strain.
A strain of Lactobacillus helveticus carrying the cryptic plasmid pCP53 was used for a detailed analysis of the survival and persistence of this organism in the faeces of volunteers administered oral doses of the strain. The CP53 strain had high af®nity for Caco-2 cells, but displayed low bile acid resistance in vitro. Rifampicin-resistant colonies could be reisolated from the faeces of four of seven subjects fed with the rifampicin-resistant CP53 derivative strain. Moreover, the recovery of cells, as estimated by plasmid analysis, was higher than that estimated by measuring the number of rifampicin-resistant cells. From these results, we conclude that the CP53 strain can survive passage through the gastrointestinal tract.
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