Survival of Lactobacillus helveticus strain CP53 in the human gastrointestinal tract

Shinoda, Tadashi; Kusuda, D.; Ishida, Yuu; Ikeda, Nagisa; Kaneko, Kyouko; Masuda, Osamu; Yamamoto, Naoyuki

doi:10.1046/j.1472-765x.2001.00870.x

Cited by 9 publications

(7 citation statements)

References 10 publications

(12 reference statements)

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“…Moreover, in previous studies of probiotic lactobacilli, the numbers of lactobacilli re‐isolated from feces were as follows: 10 6.8 cells g −1 of feces for L. casei strain Shirota [2], 10 7.7 cfu g −1 of feces for Lactobacillus sp. strain GG [3], 10 6.7 cells (one time feces) −1 for L. helveticus strain CP53‐R [4]. These strains could not be detected at a level comparable to the ingestion level, even if the lactobacilli were probiotics.…”

Section: Resultsmentioning

confidence: 94%

“…The potential use of probiotics requires the investigation of the viability of these strains in the gastrointestinal tract. In many cases, re‐isolation of a particular strain from the feces of volunteers by the culture method has demonstrated that the ingested bacterial cells can survive through the gastrointestinal tract [2–4]. However, the ratio of lactobacilli in the fecal microflora is relatively low, even though lactobacilli are major bacteria in the small intestine.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring the cell number and viability ofLactobacillus helveticusGCL1001 in human feces by PCR methods

Saito¹,

Sakamoto

Takizawa³

et al. 2004

FEMS Microbiology Letters

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Real-time polymerase chain reaction (PCR) and nested reverse transcription (RT) PCR were applied to demonstrate the viability of lactobacilli in the feces of volunteers fed fermented milk containing lactobacilli. Two sets of specific primers and a TaqMan probe for realtime PCR were constructed using the S-layer gene as a target. After fermented milk ingestion, Lactobacillus helveticus GCL1001 was detected in the feces of 12 volunteers over a few days, with the maximum number being between 10 4:5 and 10 7:8 cells g 31 of feces. Moreover, mRNA from this strain was detected in the feces of all volunteers by nested RT-PCR. The results show that these methods are applicable to the demonstration of bacterial viability in feces, and that ingested L. helveticus GCL1001 can survive through the gastrointestinal tract.

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Monitoring the cell number and viability ofLactobacillus helveticusGCL1001 in human feces by PCR methods

Saito¹,

Sakamoto

Takizawa³

et al. 2004

FEMS Microbiology Letters

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“…If the number of enterococci recovered had been decreased, greater Lactobacillus strain diversity may have been revealed. Rif r marking to recover introduced Lactobacillus strains from the indigenous GIT microbiota has been successfully used in chickens (23,43), ducks (16), pigs (22,39), mice (19,41), and humans (20,45), so a Rif r background was unexpected in this study. Rif MIC levels within several Lactobacillus and Pediococcus species have been reported to range between Յ0.016 and 4 g ml Ϫ1 (12,13).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Stephenson

Moore

Allison

2010

Appl Environ Microbiol

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Lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. The aim of this study was to use an in vivo model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated Lactobacillus strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. Chicken-derived Lactobacillus strains were genetically marked with rifampin resistance and administered on day 0 to chickens fed either a normal commercial diet or a specially formulated high-protein diet. Chickens fed the high-protein diet were also coinoculated with two different mixes of additional lactic acid bacteria. Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to identify rifampin-resistant isolates recovered from chickens. Three strains, belonging to the species Lactobacillus agilis, Lactobacillus crispatus, and Lactobacillus vaginalis, were commonly reisolated from the chickens on both diets at days 21 and 42. The ability of these strains to persist was confirmed in a second chicken trial. All three strains persisted throughout the production period in the chickens fed a commercial diet, while only the L. agilis and L. vaginalis strains persisted in the chickens fed the high-protein diet. In both in vivo trials, competing lactic acid bacteria modified representation of the strains recovered, with all three stains capable of competing in the presence of one or both mixes of coinoculated strains. The in vivo model successfully identified three persistent strains that will be characterized further.The ecology of the chicken gastrointestinal tract (GIT) has been studied in depth using both culture-dependent (5,7,21,40) and -independent methods (2,3,7,33,54). These studies have revealed that lactobacilli are autochthonous residents in chickens, where they predominate in the proximal GIT and are present but less abundant within the distal GIT (52). The most commonly identified Lactobacillus species are Lactobacillus crispatus, Lactobacillus reuteri, and Lactobacillus salivarius (1,7,15,26,28). A detailed understanding of the relationship between these bacteria and their host under different dietary and environmental conditions will facilitate the development of lactobacilli for various applications directed toward increasing broiler production efficiency and improving chicken health.Since the withdrawal of antimicrobial growth promoters from chicken feed in Europe, the incidence of necrotic enteritis (NE) has increased (9, 51). Consequently, there is a need to develop alternative methods for controlling the causative agent of NE, Clostridium perfringens, in the chicken GIT. Lactobacilli are excellent candidates for alternative control methods due to their autochthonous nature and dominance of the upper GIT microbiota, particularly within the small intestine where NE occurs. Their potential utility ...

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“…Ten microliters of the cell suspension was inoculated in a 96-well plate containing 190 μl of MRS added with different concentrations of each bile salt and then incubated for 24 h at 45°C. After incubation, Abs 600 was measured and the results were expressed as the percentage of growth in the presence of bile salts compared to the control grown without the addition of any compound (Shinoda et al, 2001).…”

Section: Methodsmentioning

confidence: 99%

Genomic Comparison of Lactobacillus helveticus Strains Highlights Probiotic Potential

et al. 2019

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Lactobacillus helveticus belongs to the large group of lactic acid bacteria (LAB), which are the major players in the fermentation of a wide range of foods. LAB are also present in the human gut, which has often been exploited as a reservoir of potential novel probiotic strains, but several parameters need to be assessed before establishing their safety and potential use for human consumption. In the present study, six L. helveticus strains isolated from natural whey cultures were analyzed for their phenotype and genotype in exopolysaccharide (EPS) production, low pH and bile salt tolerance, bile salt hydrolase (BSH) activity, and antibiotic resistance profile. In addition, a comparative genomic investigation was performed between the six newly sequenced strains and the 51 publicly available genomes of L. helveticus to define the pangenome structure. The results indicate that the newly sequenced strain UC1267 and the deposited strain DSM 20075 can be considered good candidates for gut-adapted strains due to their ability to survive in the presence of 0.2% glycocholic acid (GCA) and 1% taurocholic and taurodeoxycholic acid (TDCA). Moreover, these strains had the highest bile salt deconjugation activity among the tested L. helveticus strains. Considering the safety profile, none of these strains presented antibiotic resistance phenotypically and/or at the genome level. The pangenome analysis revealed genes specific to the new isolates, such as enzymes related to folate biosynthesis in strains UC1266 and UC1267 and an integrated phage in strain UC1035. Finally, the presence of maltose-degrading enzymes and multiple copies of 6-phospho-β-glucosidase genes in our strains indicates the capability to metabolize sugars other than lactose, which is related solely to dairy niches.

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Survival of Lactobacillus helveticus strain CP53 in the human gastrointestinal tract

Cited by 9 publications

References 10 publications

Monitoring the cell number and viability ofLactobacillus helveticusGCL1001 in human feces by PCR methods

Monitoring the cell number and viability ofLactobacillus helveticusGCL1001 in human feces by PCR methods

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Genomic Comparison of Lactobacillus helveticus Strains Highlights Probiotic Potential

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