Chemiluminescence (CL)-based technologies have revolutionized in vivo monitoring of biomolecules.H owever, significant technical hurdles have limited the achievement of trigger-controlled, bright, and enriched CL signal. Herein, ad ual-lock strategy uses sequence-dependent triggers for bright optical imaging with real-time fluorescent signal and ultra-sensitive CL signal. These probes can obtain an analytetriggered accumulation of stable pre-chemiluminophore with aggregation-induced emission (AIE), and then the pre-chemiluminophore exhibits ar apid photooxidation process (1,2dioxetane generation) by TICT-based free-radical addition, therebya chieving an enrichment and bright CL signal. The dual-locks trategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high-resolution, dual-mode of chemo-fluoro-luminescence,a nd three-dimensional (3D) imaging in living animals.
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