A Sequential Dual‐Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

Abstract

Chemiluminescence (CL)-based technologies have revolutionized in vivo monitoring of biomolecules.H owever, significant technical hurdles have limited the achievement of trigger-controlled, bright, and enriched CL signal. Herein, ad ual-lock strategy uses sequence-dependent triggers for bright optical imaging with real-time fluorescent signal and ultra-sensitive CL signal. These probes can obtain an analytetriggered accumulation of stable pre-chemiluminophore with aggregation-induced emission (AIE), and then the pre-chemiluminophore exhibits ar apid photooxidation process (1,2dioxetane generation) by TICT-based free-radical addition, therebya chieving an enrichment and bright CL signal. The dual-locks trategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high-resolution, dual-mode of chemo-fluoro-luminescence,a nd three-dimensional (3D) imaging in living animals.

Spatio‐Temporally Reporting Dose‐Dependent Chemotherapy via Uniting Dual‐Modal MRI/NIR Imaging

“…To confirm the feasibility of NQ-Cy@Fe&GOD for monitoring COH-mediated therapy,w ef irstly investigated the fluorescence response of probe NQ-Cy with endogenous NQO1 enzyme.H erein, human lung cancer cells A549 with overexpressed NQO1 enzyme and normal liver cell QSG-7701 were chosen as model cell lines. [18] As expected, the strong NIR-2 signal of NQ-Cy was only observed in normal QSG-7701 cells ( Figure 3A), while dual-channel fluorescence signal of NIR-1 and NIR-2 were both found from probe NQ-Cy incubation with A549 cells ( Figure 3B). Indeed, ah igh level of cellular NQO1 enzyme triggered the cleavage of NQ-Cy (Figure 2A)toallow an obvious emission enhancement at 650 nm (NIR-2), but concomitantly weak the initial emission at 830 nm (NIR-1).…”

A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues

“…[9][10][11][12] Currently, several analytical methods based on colorimetric assays, 10,11 chemiluminescence, 12 electrochemical sensors, 13,14 nanoparticles, 15 and supramolecular assemblies, 16 have been engineered to probe AChE activity. Fluorescent probes combined with uorescence confocal imaging with unique features, [17][18][19][20][21][22][23][24][25][26][27][28] such as in situ and/or real-time detection, high spatiotemporal resolution and noninvasive monitoring abilities, have been extensively used in various disease diagnoses and therapies. 29,30 Currently, uorescent probes for AChE activity analysis employ acetyl groups as recognition units.…”

Spatio‐Temporally Reporting Dose‐Dependent Chemotherapy via Uniting Dual‐Modal MRI/NIR Imaging

“…OH‐mediated therapy, we firstly investigated the fluorescence response of probe NQ‐Cy with endogenous NQO1 enzyme. Herein, human lung cancer cells A549 with overexpressed NQO1 enzyme and normal liver cell QSG‐7701 were chosen as model cell lines [18] . As expected, the strong NIR‐2 signal of NQ‐Cy was only observed in normal QSG‐7701 cells (Figure 3 A), while dual‐channel fluorescence signal of NIR‐1 and NIR‐2 were both found from probe NQ‐Cy incubation with A549 cells (Figure 3 B).…”