Field muskmelon seed oil was extracted by press extraction (PE), Soxhlet extraction (SE), organic extraction (OSE), and aqueous extraction (AE). The oils were then evaluated for their physicochemical properties, fatty acid composition, volatile compounds, and antioxidant properties. A high yield oil was found in the SE sample. The AE sample had the highest elevated acid and peroxide values, while PE and OSE had the highest oil iodine content. The oil samples did not differ significantly in their fatty acid profile depending on the extraction method. However, E-nose, HS-GC-IMS, and HS-SPME-GC-MS showed that the flavor composition of the four samples was significantly different, attributed to the changes in the composition and content of the compounds caused by the different extraction methods. Furthermore, the strongest FRAP and the free radical scavenging ability of DPPH and ABTS+ showed in the SE sample. In general, SE’s seed oil has certain advantages when applied to the muskmelon seed oil industry.
For oil plants, the oil extraction method is a crucial factor in influencing the functional characteristics of the protein. However, reports of protein functionality as affected by the oil extraction process are scarce. In this study, field muskmelon seed (FMS) protein was extracted by Soxhlet extraction method (SE), organic solvent extraction method (OSE), aqueous extraction method (AE), and pressing extraction method (PE), and its structure, amino acid profile, physicochemical properties, and functionality were determined. Molecular weight distribution was similar for all FMS proteins, whereas protein aggregates contents were most excellent for SE and OSE. FMS protein comprised predominantly glutamic acid, leucine, aspartic acid, arginine, and proline. Total amino acids content was highest for SE. Differences in functionality between four FMS proteins for different oil extraction methods were vast. PE had the highest value of solubility, and AE exhibited the lowest. AE had the greatest water and oil holding capacity. PE presented better foaming and emulsion capacities than other samples. This study demonstrated that the extraction oil method could impact the protein’s physicochemical and associated functional characteristics. High-quality plant oil and protein could be simultaneously obtained by modulating the oil extraction method in future research.
MYB TFs plays an important role in plant adaptation to abiotic stress, especially in response to salt stress. Preliminary research found that the stress resistance of the two melon varieties ‘M15’ and ‘baogua’ are quite different. To this end, we compared the transcriptomes of ‘M15’ and ‘baogua’. Transcriptome analysis found that the expression levels of 12 MYB transcription factors were significantly different between the two varieties.CmMYB1 gene was cloned from muskmelon (Cucumis melo L.), and the subcellular localization results showed that CmMYB1 was expressed in the cytoplasm and nucleus. Transform CmMYB1 gene into Arabidopsis thaliana, observe T3 phenotypes during the vegetative and reproductive growth periods.The results showed that the transgenic A. thaliana phenotype did not change significantly compared with the wild type . After treatment with 200 mM sodium chloride solution for 1 h and 3 h, the CmMYB1 expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. These results provide a theoretical basis for the further study of the biological function of CmMYB1.
This study aims to clone the MYB gene from the melon and explore its function. In order to obtain the differentially expressed MYB genes, comparative transcriptome analysis was carried out between varieties ‘M15’ and ‘Baogua’. CmMYB1 homologous gene in muskmelon (Cucumis melo L.) was obtained by homologous cloning. CmMYB1 gene was introduced into wild type by agrobacterium‐mediated transformation, and the growth status of T2 transgenic and wild type was observed. The results of subcellular localization showed that the CmMYB1 protein was expressed in cytoplasm and nucleus. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly, and began to decline after 6 h of stress, indicating that it functions in the early response to salt stress. In CmMYB1‐OX overexpression Arabidopsis and Arabidopsis Col‐0 treated with 200 mM NaCl solution for 14 days, the overexpression lines at L4 and L5 were seen to change more obviously, and these results suggested that the CmMYB1 gene was involved in the response to salt stress.
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