CmMYB1 gene clone from Cucumis melo and its functional roles under salt stress

Abstract

This study aims to clone the MYB gene from the melon and explore its function. In order to obtain the differentially expressed MYB genes, comparative transcriptome analysis was carried out between varieties ‘M15’ and ‘Baogua’. CmMYB1 homologous gene in muskmelon (Cucumis melo L.) was obtained by homologous cloning. CmMYB1 gene was introduced into wild type by agrobacterium‐mediated transformation, and the growth status of T2 transgenic and wild type was observed. The results of subcellular localization showed that the CmMYB1 protein was expressed in cytoplasm and nucleus. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly, and began to decline after 6 h of stress, indicating that it functions in the early response to salt stress. In CmMYB1‐OX overexpression Arabidopsis and Arabidopsis Col‐0 treated with 200 mM NaCl solution for 14 days, the overexpression lines at L4 and L5 were seen to change more obviously, and these results suggested that the CmMYB1 gene was involved in the response to salt stress.

Abiotic stress responses in melon (Cucumis melo): Emerging underlying molecular mechanisms and biotechnological advances to cope with the issue

“…In addition to tissue culture, transformation via the pollen tube pathway-based protocol has also been applied for genetic transformation in melons (Bai et al, 2020;Feng et al, 2023;Hao et al, 2011). As mentioned in the previous sections, several melon genes were found to function in response to abiotic stress conditions (e.g., CmCML13, CmLOX10, and CmMYB1) (Xing et al, 2020;Yang et al, 2020;Zhang et al, 2021). Genetic transformation/editing can be used to manipulate these genes in melon plants to test whether they can improve stress tolerance in this crop species.…”

“…Interestingly, although salt stress decreased fruit yield, it increased fruit sweetness (Akrami & Arzani, 2019; Kaya et al, 2007). At least two melon ( Cucumis melo ) genes, CmCML13 and CmMYB1 have been shown to regulate salt stress responses (Yang et al, 2020; Zhang et al, 2021). Because of the difficulty in generating melon transgenic lines and mutants, these studies only reached the stage of ‘heterologously’ characterizing these genes ( CmCML13 and CmMYB1 ) by overexpression in the model plant Arabidopsis thaliana (Yang et al, 2020; Zhang et al, 2021).…”