This study aims to determine the polymorphism of Prolactin (PRL | XbaI and PRL | DraI) genes in Bayang ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) technique and its association with weight of ducks aged 1-10 weeks. This study used 200 Bayang duck blood samples consisting of 102 male ducks (♂) and 98 female ducks (♀). DNA from blood samples was isolated using the Genomic DNA Purifification Kit (Promega) using protocol from the manucfacture. The DNA was then amplified using two primers with F: 5′-AAA TTC CCT CTC ACA GTT ACA-3′ and R: 5′-GAT GCA GAG ACA AGT TTC ACC-3′ and F: 5′-GAATAGAACACTTGACCCTG-3′ and R: 5′-TAGAGGAGGCAAGCATAG-3′ which produces fragments with a length of 416 bp and 566 bp. Restriction with XbaI enzymes that recognized the binding site (5-TT CTAGA-3′) resulted 3 genotypes: homozygote (+/+), heterozygote (+/−) and homozygote (−/−) with frequencies 0.455, 0.495 and 0.050 respectively and with frequency allel (+) 0.702 and frequency allel (−) 0.297. While the results of the restriction with enzyme DraI found three types of genotypes, namely (+/+), (+/−) and (−/−) with frequencies of 0.64, 0.35 and 0.01 respectively with frequency allel (+) 0.82 and allele frequencies (−) 0.18. From the results of the analysis, it was found that there was no relationship between these two diversity and weight ducks of duck.
Polymorphisms in the growth hormone (GH) gene that is associated with the growth rate of duck have been the target of many breeding programmes. Molecular market selection has been an acceptable tool in the acceleration of the genetic response of desired to improve production performance in livestock. Growth hormone (GH) has been considered as a candidate gene for growth traits. In this study, polymorphisms of the GH gene were evaluated for associations with body weight in 210 Bayang duck. The objective of this study was to assess the association of polymorphisms in Growth Hormone (GH) genes with growth in Bayang ducks. The polymorphism of the ducks GH gene from promoter region into exon 3 was researched by polymerase chain reaction and DNA sequencing methods. Fifty duck were genotyped by sequencing twenty mutations were detected in the Bayang duck GH gene. Mutation G→A were detected at position -142, 1155, 1117, 1423, and 1786. Mutation C→T were detected at position -62, 506, and 308. Mutation A→G were detected at position 160, 264, 293, 1245, and 2542. Mutation T→G detected at position 250 and mutation TT→AA detected at position 350. Mutation G→T detected at position 775 and mutation T→C were detected at position 1353, 1424, and 2973. All polymorphism were polymorphics except polymorphism T→G at position 250 was monomorphics. Mutation G→A in position 1117 and 1786 were restriction with enzyme Eco721 and TscaI. In the case GH-Eco721 three genotypes were observed, GG, GA, and AA with frequency 0.041, 0.59 and 0.369 respectively. For GH-TscaI locus the frequency were 0.034, 0.782, and 0.184 respectively. The effect of GH-TscaI polymorphism were observed on body weight in 4 to 8 week of Bayang duck.
The objective of this research was to obtain quantitative growth hormone gene polymorphism association between growth hormone gene genotype with quantitative characteristics of the thin-tail sheep in the highlands and lowlands of Jambi Province. Two phases of research were done on the field and in the laboratory. Field research which was conducted to obtain the quantitative characteristics data includes; withers height (WH), body length (BL), chest grid (ChG), chest depth (ChD), chest width (ChW), body weight (BW) and body weight gain (BWG). Laboratory research included: DNA isolation, amplification and gel purification, characterization and identification using PCR-RFLP with the MspI, and AluI. Quantitative characteristics data was collected from 240 heads of thin-tailed sheep. Blood samples were collected from 160 of them and all were between the ages of 1 and 2 years (I1 = pair of permanent teeth). Field research was conducted in the Kerinci District and Sungai Penuh City (Highlands) and Muara Jambi Districts and Batanghari Districts (lowlands). The purposive sampling technique used in this research revealed that: 1) quantitative characteristics (BW, BWG, BL, ChG, ChD, and ChW) of thin-tailed sheep both male and female in highland were better than in the lowlands; 2) studied locus was polymorphic on the highlands and lowlands; 3) the highest of genotype and allele frequencies of both highlands and lowlands were related to the genotype (+/+) and allele (+), respectively; 4) the highest quantitative characteristic (BW BW, BWG, BL, ChG, ChD, and ChW) of thintailed sheep was genotype (+/+); and 5) the diversity of GH genes MspI and AluI were associated with BW, BWG, BL, ChG, ChD, and ChW of thin-tailed sheep both on the highlands and lowlands of Jambi Province.
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