Polymorphisms of Growth Hormone Gene Exon 1 and their Associations with Body Weight in Pitalah and Kumbang Janti Ducks

Yurnalis,; Husmaini, H; Sabrina,

doi:10.3923/ijps.2017.203.208

Cited by 3 publications

(2 citation statements)

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“…A number of 33 SNPs in GHR gene was reported by Nie et al (2005), and the g.565G>A GHR polymorphism was reported had significant associations with fatness and muscle fiber traits in Chinese local chickens (Lei et al, 2007). Studies in other poultries reported the associations of the GH gene with growth and carcass traits in Pitalah and Kumbang Janti Indonesian ducks (Yurnalis et al, 2017), Huoyan Chinese native goose , and Japanese quail (Johari et al, 2013). In other farm animals, the GH gene was reported in cattle breeds (Dolmatova & Ilyasov, 2011), pig (Ashok et al, 2014), salmonids fish (Kamenskaya et al, 2015), sheep (Jia et al, 2014), goat , camel (Shawki et al, 2015), and passerine bird (Arai & Iigo, 2010).…”

Section: Introductionmentioning

confidence: 99%

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

Khaerunnisa

Jakaria

Arief

et al. 2017

Med Pet

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The chicken growth hormone (GH) and its receptor (growth hormone receptor, GHR) play important roles in chicken performances due to their crucial functions in growth. The variations of GH and GHR genes were then thought to be associated with the variations of the performances. This experiment was designed to identify the g.2248G>A GH and the g.565G>A GHR loci polymorphisms and to evaluate their associations with carcass components in Kampung and broiler chicken cross. A total of 215 chickens including 4 chicken populations (Kampung, Cobb broiler, F1, and F2 Kampung x broiler chicken cross) were screened to identify polymorphism using PCR-RFLP technique with EcoRV and Eco72I restriction enzyme for GH and GHR loci, respectively. The carcass components were recorded at 26 weeks of age on F2 Kampung and broiler chicken cross (42 chickens) for association study. Both the g.2248G>A GH and the g.565G>A GHR loci were polymorphic with two alleles (G and A) and three genotypes (GG, AG, and AA). The GG genotype and the G allele of GH locus were predominant in all chicken populations. While in GHR locus, the AA genotype and the A allele were found to be higher in all chicken populations. The association study showed that the g.565G>A GHR locus polymorphism had significant effect on carcass components, including live weight, carcass weight, breast weight, thighs weight, breast muscle weight, and thighs muscle weight. There was no significant association was found between the g.2248G>A GH genotype and carcass components. It could be concluded that the g.2248G>A GH and the g.565G>A GHR loci were polymorphic in Kampung and broiler chicken cross and the g.565G>A GHR locus was accosiated with carcass components. This g.565G>A GHR SNP might be an important candidate marker for chicken growth and muscle mass improvement. Keywords: Kampung chicken cross, growth hormone, growth hormone receptor, gene polymorphisms, carcass ABSTRAKHormon pertumbuhan dan reseptor hormon pertumbuhan memiliki peran penting dalam performa pertumbuhan ayam. Tujuan penelitian ini adalah mengidentifikasi keragaman gen hormon pertumbuhan (lokus g.2248G>A GH) dan gen reseptor hormon pertumbuhan (lokus g.565G>A GHR) dan mengevaluasi pengaruhnya pada komponen karkas pada ayam silangan Kampung dan broiler. Penentuan genotipe dilakukan pada 215 ekor ayam, yang terdiri atas 4 populasi (Kampung, ras pedaging strain Cobb, silangan F1 dan F2 Kampung x ras pedaging strain Cobb) menggunakan enzim pemotong EcoRV dan Eco72I masing-masing untuk fragmen lokus g.2248G>A GH dan lokus g.565G>A GHR. Analisis asosiasi dengan komponen karkas dilakukan pada populasi ayam silangan F2 Kampung x ras pedaging strain Cobb umur 26 minggu. Kedua lokus tersebut bersifat polimorfik dengan dua alel yang ditemukan (alel A dan G) dan tiga genotipe (genotipe GG, AG, dan AA). Genotipe GG dan alel G pada lokus g.2248G>A GH ditemukan dalam frekuensi tertinggi di semua populasi yang diuji. Sementara pada lokus g.565G>A GHR, genotipe AA dan alel A ditemukan paling dominan. Analisis asosiasi...

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Section: Introductionmentioning

confidence: 99%

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

Khaerunnisa

Jakaria

Arief

et al. 2017

Med Pet

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“…Growth, carcass quality, meat quality, and reproductive qualities are just a few of the traits that have been linked to genes or markers through advancements in the science of molecular genetics in livestock animals [4].…”

Section: Introductionmentioning

confidence: 99%

The Relationship Between Body Weight and Growth Hormone Gene Polymorphism in Indigenous Turkeys of Nigeria

Oso,

Akinyemi,

Osaiyuwu

et al. 2024

J. Exp. Agric. Int.

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The candidate gene approach is a valuable tool for improving breeding programs, particularly in poultry, by identifying traits for enhanced production performance. This study investigates the growth hormone (GH) gene as a potential determinant of body weight in Nigerian indigenous turkeys. DNA was extracted from 60 twelve-week-old turkeys using blood samples, and a 466bp DNA fragment was amplified via PCR with primers designed from chicken sequences. The PCR product underwent Msp1 restriction enzyme digestion and was visualized on a 1.5% agarose gel. Genotypes associated with body weight were assessed using a General Linear Model. The GH/Msp1 locus revealed Two alleles (A and G) and three genotypes (AA, AG, and GG), with allele G being predominant. Genotype AG had the highest frequency (0.55), followed by AA (0.42), and GG had the lowest frequency (0.03). However, the least square means analysis showed that the polymorphism in the GH gene of the studied population was not significantly associated with body weight (p> 0.05). Nonetheless, the polymorphic nature of the GH gene in Nigerian turkeys suggests potential for further exploration in quantitative traits.

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Identification of GH Gene Polymorphisms and Their Association with Body Weight in Bayang Duck, Local Duck from West Sumatra, Indonesia

Yurnalis

Arnim

Putra

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

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Polymorphisms in the growth hormone (GH) gene that is associated with the growth rate of duck have been the target of many breeding programmes. Molecular market selection has been an acceptable tool in the acceleration of the genetic response of desired to improve production performance in livestock. Growth hormone (GH) has been considered as a candidate gene for growth traits. In this study, polymorphisms of the GH gene were evaluated for associations with body weight in 210 Bayang duck. The objective of this study was to assess the association of polymorphisms in Growth Hormone (GH) genes with growth in Bayang ducks. The polymorphism of the ducks GH gene from promoter region into exon 3 was researched by polymerase chain reaction and DNA sequencing methods. Fifty duck were genotyped by sequencing twenty mutations were detected in the Bayang duck GH gene. Mutation G→A were detected at position -142, 1155, 1117, 1423, and 1786. Mutation C→T were detected at position -62, 506, and 308. Mutation A→G were detected at position 160, 264, 293, 1245, and 2542. Mutation T→G detected at position 250 and mutation TT→AA detected at position 350. Mutation G→T detected at position 775 and mutation T→C were detected at position 1353, 1424, and 2973. All polymorphism were polymorphics except polymorphism T→G at position 250 was monomorphics. Mutation G→A in position 1117 and 1786 were restriction with enzyme Eco721 and TscaI. In the case GH-Eco721 three genotypes were observed, GG, GA, and AA with frequency 0.041, 0.59 and 0.369 respectively. For GH-TscaI locus the frequency were 0.034, 0.782, and 0.184 respectively. The effect of GH-TscaI polymorphism were observed on body weight in 4 to 8 week of Bayang duck.

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Polymorphisms of Growth Hormone Gene Exon 1 and their Associations with Body Weight in Pitalah and Kumbang Janti Ducks

Cited by 3 publications

References 19 publications

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

The Relationship Between Body Weight and Growth Hormone Gene Polymorphism in Indigenous Turkeys of Nigeria

Identification of GH Gene Polymorphisms and Their Association with Body Weight in Bayang Duck, Local Duck from West Sumatra, Indonesia

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