A study of the influence of chronic alcohol intoxication, constant illumination and their combined effects on the morphofunctional state of the rat liver and the circadian rhythms (CR) of the studied parameters of the organism was carried out. It was found that both alcohol and constant illumination caused significant changes in the structure of the liver, as well as in the circadian rhythmicity of micromorphometric parameters of hepatocytes, ALT, and total and direct bilirubin rhythms; however, the combined effects of ethanol and constant illumination had the most significant effect on the studied parameters of the organism. These two factors caused disturbances in the circadian rhythms of the micromorphometric parameters of hepatocytes, disruption of the circadian rhythms of total protein, albumin, AST, ALT, and direct and total bilirubin, as well as disturbances in the expression and rhythmicity of the studied clock genes against a background of the development of an inflammatory process in the liver.
The article discusses the experience of using the iSpring Suite 9 computer platform as an information tool for conducting distance classes with second-year students in the training for “Business Informatics”. Self-isolation requirements in the face of the COVID-19 pandemic have required the development and use of innovative remote learning methods. The need to conduct practical classes on solving technical problems with mandatory time control and prompt receipt of the results of the solution by the teacher determined the requirements for the choice of a computer program as a tool for providing distance work in the field of education. As a result, the choice was made in favor of the corporate training platform iSpring. According to the results of the spring semester, the experience of working with iSpring Suite 9 in creating electronic tests and conducting practical exercises in distance mode was recognized as successful and will be used in the future to conduct classes with students of the distance department of Novosibirsk State Technical University.
The possibilities of using optical spectroscopy methods in the differential diagnosis of prostate cancer were investigated. Analytical discrimination models of Raman spectra of prostate tissue were constructed by using the projections onto latent structures data analysis(PLS-DA) method for different wavelengths of exciting radiation—532 and 785 nm. These models allowed us to divide the Raman spectra of prostate cancer and the spectra of hyperplasia sites for validation datasets with the accuracy of 70–80%, depending on the specificity value. Meanwhile, for the calibration datasets, the accuracy values reached 100% for the excitation of a laser with a wavelength of 785 nm. Due to the registration of Raman “fingerprints”, the main features of cellular metabolism occurring in the tissue of a malignant prostate tumor were confirmed, namely the absence of aerobic glycolysis, over-expression of markers (FASN, SREBP1, stearoyl-CoA desaturase, etc.), and a strong increase in the concentration of cholesterol and its esters, as well as fatty acids and glutamic acid. The presence of an ensemble of Raman peaks with increased intensity, inherent in fatty acid, beta-glucose, glutamic acid, and cholesterol, is a fundamental factor for the identification of prostate cancer.
The aim of the research was to study the effect of chronic alcohol intoxication and constant il-lumination on the circadian rhythms (CR) of some parameters of the cardiovascular system in rats separately, as well as to study the rhythms of these parameters under the combined action of chronic alcohol intoxication (CAI) and constant illumination. It was found that chronic alcohol intoxication CAI at a fixed light regime causes a decrease in heart rate, an increase in SBP and PP; no changes were noted at CAI under constant lighting. At the same time, constant illumina-tion without ethanol exposure results in a decrease in heart rate and an increase in PP. At the same time, CAI with a fixed light regime leads to the destruction of CR of all parameters, except for MBP; at constant illumination with CAI no circadian rhythms of HR, DBP, PP and MBP are detected. Constant illumination leads to the destruction of the CR of PP. Among the remaining CRs, the heart rate rhythm, which is extant in the second group, persists practically unchanged, but the characteristics of all other CRs change significantly in comparison with control.
Wistar rats in the reproductive period and the period of pronounced senile changes in norm and pathological conditions were studied. The same informational parameters were also determined for some non-oncological pathologies, for hepatocellular adenoma and for hepatocellular carcinoma at both ages. It is established that in ontogenesis there is an increase in the level of real structural diversity (Н) and coefficient of relative entropy of the system (h). At the same time a decrease of level of organization of system (S) and coefficient of relative organization of system (R) are noted. In the studied periods of ontogenesis, at similar pathologies and pathological processes in liver, in reproductive period of ontogenesis the organ is characterized by fewer devia-tions from the norm and higher level of adaptation and compensative abilities than in the period of pronounced senile changes. Changes in informational condition of liver at hepatitis have the same direction as ontogenetic changes. They lead to an increase in the disorder of the system and a decrease in the level of its integrity, which results in a decrease in the level of adaptive and compensatory resources. In tumors, a decrease in H and h was found with a simultaneous increase in S and R compared to age norms, which are more pronounced in malignant neoplasms. For all the studied pathologies, changes in the information state are more prominent in the liver of rats in the period of pronounced senile changes.
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