A mutant strain characterized by hyperproduction of a number of cell wall and supernatant proteins was isolated after N‐methyl‐N′‐nitro‐N‐nitrosoguanidine treatment of Listeria monocytogenes strain NCTC10527. Several of these proteins were identified as virulence factors. When a wild‐type strain was grown in the presence of activated charcoal it was shown to exhibit the same protein pattern as the isolated mutant.

No abstract

A method for identification of Listeria in food samples was developed. It consisted of cultivating of suspected specimen on standard agar medium, direct absorption of grown colonies onto nitrocellulose membrane and processing of the latter with rabbit serum raised against purified cell wall protein Lm79/39 of L. monocytogenes. Analysis using anti-rabbit peroxidase conjugate and 4-chloro-1-naphthol and H2O2 solutions allowed direct detection of Listeria colonies which remained readily available for subsequent isolation.

No abstract

No abstract

No abstract

No abstract

No abstract