As the strategies of enzyme immobilization possess attractive advantages that contribute to realizing recovery or reuse of enzymes and improving their stability, they have become one of the most desirable techniques in industrial catalysis, biosensing, and biomedicine. Among them, 3D printing is the emerging and most potential enzyme immobilization strategy. The main advantages of 3D printing strategies for enzyme immobilization are that they can directly produce complex channel structures at low cost, and the printed scaffolds with immobilized enzymes can be completely modified just by changing the original design graphics. In this review, a comprehensive set of developments in the fields of 3D printing techniques, materials, and strategies for enzyme immobilization and the potential applications in industry and biomedicine are summarized. In addition, we put forward some challenges and possible solutions for the development of this field and some possible development directions in the future.
Biomedical dressings have been comprehensively explored for wound healing; however, the complicated manufacturing process and mono-function of the dressing remain critical challenges for further applications. Here, a versatile extrusion three-dimensional (3D) printing strategy to prepare MXene and spidroin-incorporated microneedle scaffolds with photothermal responsive and self-healing properties for promoting wound healing is proposed. Inspired by the cactus, the microneedle scaffold is composed of a top porous scaffold, and microneedles whose inverse opal (IO) photonic crystal (PC) structure and the ample space between the scaffold gaps endow the microneedle scaffold with high drug-carrying capacity. Furthermore, the excellent electrical and photothermal properties of MXene allow the microneedle scaffold to perform sensitive wound movement monitoring and controlled drug release under nearinfrared irradiation. Moreover, the extensive hydrogen bonding and Schiff base between the spidroin, polyurethane (PU), and aloe vera gel (avGel) molecules conferred high self-healing and mechanical performance to the microneedle scaffold. In vivo experiments with rat models of wounds have shown that drug-laden microneedle scaffolds under near-infrared (NIR) light can promote the recovery of full-skin wounds. These unique characteristics suggest that 3D-printed multifunctional microneedle scaffolds show great potential for applications in facilitating wound healing and will find widespread applications in wound management.
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