Introduction: The massive hepatic necrosis of acute liver failure (ALF) results in a sudden loss of hepatic cells. Although most hepatocyte cells of ALF are completely lost, stem cell-derived circulating cells and endogenous progenitor cells rapidly regenerate them. Mesenchymal stem cells (MSCs) have a critical role in the regeneration of liver injury through regulating platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) levels. However, their fluctuating levels in the healing process and correlation to the decrease of liver function markers remain unclear. The aim of this study was to analyze the effects of MSCs in accelerating liver regeneration of ALF by measuring VEGF and PDGF levels on day 2 and 7, as well as SGPT and SGOT levels, and assessing histopathology appearance. Methods: Using an ALF rat model, 12 animals were randomly assigned into two groups: umbilical cord (UC)-MSC injection (T1) and vehicle control (Veh). ELISA assay was employed to measure PDGF and VEGF levels, an automatic analyzer was used to assess serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT), and hematoxylin and eosin (H&E) staining was used to evaluate morphological appearance. Results: The study showed an significant (P<0.001) increase of PDGF and VEGF levels on the 2nd day, followed by a decrease on the 7th day, along with a decrease of SGPT and SGOT levels as well as the normality of histology appearance. Conclusion: In conclusion, administration of MSCs may accelerate liver regeneration of ALF through PDGF and VEGF regulation.
Pregnancy is a maturation crisis that can be stressful. The maturation crisis can be overcome if the mother is ready to enter a new phase where she is ready to take on the responsibilities of being a parent and a new role so that the mother must take care of her health to prevent complications of pregnancy. Pregnancy complications can be prevented by maintaining the distance of pregnancy between one child with another child through postpartum contraception. The study aimed to determine the characteristics of pregnant women who have sufficient and good knowledge of the use of postpartum contraception. Quantitative research with an exploratory descriptive approach has been carried out on 34 pregnant women who performed antenatal care at the Kedung 2 Jepara Public Health Center. The data collection tool uses a questionnaire. Data were analyzed by univariate analysis using frequency distribution and bivariate analysis using test chi-square. The results showed that the average age of pregnant women was 29 years. The majority of pregnant women had the last high school education, work, and the average income was Rp. 1,500,000/month. The majority of pregnant women had one child and when data were collected for pregnant women who were 1st and 2nd pregnant status. The majority of pregnant women had a good level of knowledge about the use of postpartum injection contraception. The results of the chi-square test analysis showed that the characteristics of pregnant women that had a significant relationship with knowledge about the use of postpartum contraception were age (p = 0.027), education (p = 0.004), occupation (p = 0.002), number of children (p = 0.006). Meanwhile, income did not have a significant relation with maternal knowledge in using postpartum contraception (p = 0.923).
Hemoglobin (Hb) level is a biochemical indicator of the nutritional status of pregnant women, and a low Hb level reduces blood supply to the placenta, which influences the incidence of placenta previa. A placenta previa develops in the lower portion of the uterus, completely or partially expanding to cover the entire birth canal and the internal uterine ostium. The APGAR score is commonly used as a predictor of infant mortality and long-term disability and as an indicator of infant health at birth. The risk of maternal and infant mortality and morbidity increases in placenta previa patients with inadequate vascularization, which can be caused by low Hb levels. This study aimed to determine the relationship between Hb levels and APGAR scores in patients with placenta previa at Sultan Agung Islamic Hospital in Semarang. This analytic observation study is a cross-sectional analysis with purposive sampling method. From January 2017 to January 2018, all placenta previa patients hospitalized at Sultan Agung Hospital in Semarang were included in this study. In this study, we used subject data that met the criteria for inclusion. The p-value derived from the chi-square analysis is 0.041 (p< 0.05). A test of association was conducted. This test revealed that the OR was 11.5 (OR > 1). Low Hb levels (11 g/dl) could significantly increase the risk of a lower APGAR score at 5 min in patients with placenta previa who have a low Hb level.
Mesenchymal stem cell (MSC) mempunyai kemampuan immunoregulasi dan regenerasi melalui supresi pelepasan mediator proinflamasidan peningkatan molekul proliferasi terutama vascular endothelial growth factor (VEGF) dan platelet-derived growth factor (PDGF). Mesenchymal stem cell yang diaktivasi TNF-α dengan dosis tertentu mampu meningkatkan sekresi VEGF dan PDGF, namun dosis optimal TNF-α yang mampu memaksimalkan ekspresi molekul tersebut belum diketahui secara pasti. Variasi dosis TNF-α digunakan pada penelitian ini dengan tujuan mengetahui dosis optimal, rendah, dan tinggi TNF-α dalam memaksimalkan ekspresi VEGF dan PDGF. Penelitian ini mengunakan post-test only control group design dengan 5 kelompok penelitian, terdiri atas satu kelompok kontrol (K) dan 4 kelompok perlakuan (P) (TNF-α= 5, 10, 40, 80 ng/mL) yang diinduksikan pada MSC dengan inkubasi 24 jam, kemudian kadar PDGF dan VEGF diukur dengan metode ELISA. Penelitian ini dilakukan antara
Tujuan : Mendapatkan gambaran luaran persalinan sungsang di RSUP Dr. Kariadi Semarang tahun 2013. Metode : Penelitian deskriptif retrospektif dan analytical work, pada 283 kehamilan dengan letak sungsang yang melahirkan di RSUP Dr. Kariadi Semarang di tahun 2013, dibagi menjadi persalinan pervaginam 129 subyek dan bedah sesar 154 subyek. Data diambil dari rekam medik pasien yang meliputi usia, pendidikan, pekerjaan, pembiayaan, usia kehamilan, paritas, hemoglobin (Hb), riwayat ANC, status dan asal rujukan, jenis persalinan, penolong persalinan, tempat persalinan, ketuban pecah dini, mortalitas neonatus, mortalitas dan morbiditas maternal. Hasil : Persalinan sungsang pervaginam memiliki risiko 5,16 kali lebih besar terjadi perdarahan post partum dan 5,32 kali lebih besar skor apgar menit ke 5 kurang dari tujuh dibanding persalinan sungsang bedah sesar. Persalinan sungsang pervaginam memiliki risiko kematian neonatus dini 3,85 kali lebih besar ketimbang persalinan sungsang bedah sesar (p=0,015; OR=3,85; IK 95% 1,21-12,23). Nulipara memiliki resiko 8,15 kali lebih besar terjadi kematian neonatal dini dibanding multipara. Simpulan : Persalinan sungsang pervaginam memiliki risiko lebih besar dibandingkan bedah sesar. Bedah sesar menawarkan hasil luaran yang lebih baik dibanding pervaginam dalam hal perdarahan post partum sementara kejadian asfiksia dan kematian neonatus dini tidak dipengaruhi oleh jenis persalinan
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