Simple, accurate, and precise spectrophotometric method has been developed and validated for the estimation of amlodipine besylate (AML) in tablets. The method is based on the condensation of AML with sodium 1,2-naphthoquinone-4-sulphonate in an alkaline medium when the reaction mixture was heated at 363 K for 1 min to form an orange-colored product. The spectrophotometric method involved the measurement of the colored product at 459 nm. The reaction conditions were studied and optimized. Beer's law was obeyed in the concentration range of 10-20 µg•ml-1 with RSD of 0.825 and 0.559 % and molar absorption of 2.54•10 4 , the range of methods application is 67-133 % of the nominal content of amlodipine besylate in the dosage forms.
In this investigation a visible spectrophotometric method for the determination of sotalol based on the absorbance of colored product of the reaction between sotalol hydrochloride and bromcresol purple in acetone medium at 399 nm measurement was developed. The optimal conditions for the quantitative determination of sotalol hydrochloride in the content of pharmaceutical drugs were established. The stoichiometric relationship coefficients between sotalol hydrochloride and bromcresol purple were determined. The validation of the worked out procedure on such validated characteristics as linearity, precision, accuracy and robustness was carried out. The aim. To develop a highly sensitive, easy to use, cost-effective and valid method for quantitative determination of sotalol hydrochloride in dosage forms. The analysis method. Visible spectrophotometry. The analytical parameters such as molar absorptivity, Beer’s law limits and Sandell’s sensitivity values were calculated. The developed methods give the result with repeatability sufficient for dependable determination the investigated substance in pharmaceutical formulations. Accuracy established by analyte addition technique. Determined factors that influence on the absorbance value: reagent quantity and timing stability. Sample solutions stable during 30 min. Addition to sample solution ± 10% bromcresol purple solution is not change the absorbance value. Established that reaction between sotalol hydrochloride and bromcresol purple proceeds in acetone medium at room temperature. Molar absorption coefficient is 2,62∙103.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.