Surya DH, Mariyah Y, Tahono. 2009. The influence of noni fruit (Morinda citrifolia) extract toward the level of SGOT and SGPT enzymes on white mice induced by carbon tetrachloride. Biofarmasi 7: 87-93. Noni fruit (Morinda citrifolia L.) is a wellknown crop in the society. It is determined for its contents including proxeronine and some antioxidants, i.e. ascorbic acid and beta-carotene that function to maintain and improve cell function. This research used hepatic cells considering the vital function of the hepatic organ in the body. The purpose of this research was to determine the effect of noni fruit extract to reduce hepatic cells damage induced by CCl4. This research was included into laboratory experimental research and used a completely randomized design. The samples consisted of 25 male white mice (Mus musculus) type Swiss Webster with the age between 3-4 months and the weight between 20-30 grams, and divided into 5 groups. The first group was the CCl4 control group, in which white mice were given toxic dosage of CCl4 11 mg/20 g BW without noni fruit extract treatment. The second group consisted of white mice given by noni fruit extract in dosage of 0.56 g/20 g BW for 8 days, and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The third group consisted of white mice given by noni fruit extract in dosage of 1.12 g/20 g BW for 8 days, and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The fourth group consisted of white mice given by noni fruit extract in dosage of 2.24 g/20 g BW for 8 days and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The fifth group consisted of white mice given only water and daily food for 8 days. Blood samples from all white mice were taken after 24 hours to determine the level of SGOT and SGPT enzymes. The results were analyzed by using One-Way Anova statistical test, which continued with Post Hoc Test and Tukey Test. The result of research showed that noni fruit extract in dosage of 0.56, 1.12, and 2.24 g/20 g BW given per oral could reduce SGOT level in 214.48±48.804 U/I, 151.16±22.811 U/I, and 169.62±44.891 U/l, respectively, compared with a positive control of CCl4 that was 296.62±59.254 U/I. Meanwhile, SGPT level became 55.42±4.292, 54.34±6.896, 58.58±8.210 U/I, compared with a positive control of CCl4 that was 83.96±2.931 U/I.
Basil leaves are believed by community to reduce the fever. This study aimed to determine the effect of antipyretic and the effectiveness of basil leaf extract (Ocimi sancti folium) in rats. This research was a laboratory experimental research by using a random sampling technique. The experimental animal used was Wistar rats aged less than 2 months, as much as 20 rats divided into 4 groups. Group 1 as a negative control group was given aquadest of 2.5 ml, while groups 2, 3 and 4 as test groups were each given basil extract by 3.15 mg/100 gr BB, 6.30 mg mg/100 gr BB and 12.60 mg/100 gr BB, respectively, dissolved in 2.5 ml of aquadest. To increase the temperature, it was injected vaccine DPT 0.2 cc intra-muscular. The temperature measurement is conducted by using a digital thermometer. The data obtained were analyzed by using Statistical Package for Social Sciences 11.5 (SPSS 11.5) with Univariate Analysis of Variance method followed by Post Hoc test. The result of one factor analysis from Univariate Analysis of Variance test showed that the average of rats rectal temperature was significantly different for each test group and for each time range of temperature measurement, each of them was 0.034 and 0.005 with a significant level (α) = 0.05. From the result of analysis by using Post Hoc Test, there was a significant difference between dose group of 3.15 mg/100 gr BB with a dose group of 12.60 mg/100 gr BB and between the time of temperature measurements of 120 minutes and 180 minutes. At first dose and 180th minute, it was obtained a lowest rectal rats temperature. The results showed that the basil leaf extract had an antipyretic effect on rats. The optimal effectiveness evidenced by a lowest average rectal temperature was achieved by a dose of 3.15 mg/100 gr BB at 180th minute.
Pengaruh ekstrak herba meniran (Phyllantus niruri) terhadap kadar trigliserida darah tikus putihThe effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats . The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1 st and 21 st , blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.
Sentana OM, Haryati S, Mariyah Y. 2011. Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro. Biofarmasi 9: 1-6. This research aimed to examine the relationship between the ethanol extract of basil leaf against Ascaris suum mortality rate in vitro. This study was a laboratory experiment with a research design of the post-test with controlled group design. The samples were Ascaris suum Goeze that still actively moved and obtained from pig intestines. The sampling technique used was a purposive sampling by equating the type and the length of worms, and it did not distinguished for the sex of worms. The subjects were divided into five groups, each group consisted of 5 worms and repeated in 6 times. The control group contained 25 mL of physiological saline solution and 25 mL pyrantel pamoate 5 mg/mL. Three other groups contained basil leaves extracts consisted of various concentrations, i.e. 30%, 40% and 50%. The number of dead worms was counted every 2 hours until all the worms in the group died. The data were analyzed by using a linear regression analysis and a probit analysis. Simple linear regression statistical test showed a significance correlation at 0.000 with 0.837 correlation coefficient was negative, R square 0.701, in which the significant correlation <0.05 or the value of 37.565 with a significance of means, there was a negative relationship between the length of time of death of worms with large concentrations of basil extract. Analysis using a probit analysis obtained LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours. The ethanol extract of basil leaf could affect the death of A. suum in vitro with LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours.
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