A convenient way of producing effective antibody against maize seed storage proteins is present.The protein patterns of maize hybrid seed Ludan50 have two special protein bands with Polyacrylamide gel electrophoresis (PAGE) which was corresponding to its parent inbred lines Luyuan92 and Qi319. We purified the two proteins respectively by acid-PAGE and then immunized rabbit and laying hens, obtained the antiserums and yolk antibodies. Both antiserums and yolk antibodies have good specificity against the antigens, except that the yolk antibody has a higher titer. We also measured its specificity against different protein components extracted with pure water, 2.5% NaCl (w/v) , 70%(v/v) alcohol , 10mM HCl and 50mM KOH. The results appears that the water-soluble proteins tended to be more specific than othors to the antibodies, so the two inbred lines can be identified from each other by ELISA with the antibody we have been produced.
The objective of the study was to optimize the conditions in a culture medium for the selenium yeild enriched by Saccharomyces sp. III using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the significant factors in the fermentation for the selenium yeild, and subsequent use of the response surface methodology was further optimized for the selenium yeild by Box-Behnken design. The important factors in the culture medium, identified by the initial screening method of Placket-Burman, were sodium selenite, glucose and the liquid volume. The optimal amounts for maximum selenium yeild were: sodium selenite 15.8 mg/L; glucose 40.2 g/L; the liquid volume 120 mL in 250 mL flask. Using this statistical experimental design, the selenium yeild under optimal condition reached about 1679.32 μg selenium /g dry cell.
Designing of a new type of metal wire-buckling machine, introducing the overall structure, operational principle and control flow of the equipment. The machine can realize the feeding, straightening, cutting off, shaping, returning material procedures of the metal wire automatically, with features of high degree of automation, compact structure, good quality and high efficiency.
Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.
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