Vascular remodeling due to hypertension is one of the major health challenges facing countries around the world. Neohesperidin, a flavonoid glycoside found in citrus fruits, is an antioxidant. Neohesperidin has been studied for a variety of diseases in addition to hypertension. In this study, angiotensin II was used to induce hypertension in mice (490 ng/kg/min, 14 days). We used H&E, Masson, immunofluorescence, dihydroethidine and qPCR to evaluate the effect of Nehesperidin (50 mg/kg/day, 16 days) on pathological hypertension in mice. Estimating the effect of Nehesperidin on human umbilical vein endothelial cells and vascular smooth muscle cells stimulated by angiotensin II. We found that neohesperidin inhibited angiotensin II-induced hypertension in mice. Neohesperidin reduced angiotensin II-induced vascular hypertrophy, fibrosis, inflammation and oxidative stress in vivo. Neohesperidin inhibited angiotensin II-induced ROS and DNA damage in human umbilical vein endothelial cells. Neohesperidin inhibited angiotensin II-induced migration of vascular smooth muscle cells. The results showed that Nehesperidin acts as an antioxidant and could significantly inhibit angiotensin II induced hypertension and vascular remodeling in vitro and in vivo.
The surface resonance scattering of silver nanoparticles, commonly used to enhance the matrix of the Raman, is the most active of all metal nano particles which can greatly improve the sensitivity of the detected molecules. At the same time, the sterilization ability of silver is also
highly recommended in the field of biology. In this study, cytosine nucleoside was used as a template to quickly synthesize silver-based composite nanomaterials (Ag–C/Au) with enhanced fluorescence and good water solubility. Then, the fluorescence spectrum, lifetime, the ultraviolet-visible
absorption spectrum of the material, and the size and shape of the grain size were studied through characterization. After that, the interaction between the silver-based composite nanomaterial Ag–C/Au and the recombinant protein ephrin-b2 was studied by spectroscopy, and the mixed metalloprotein
was used for electrocardiogram treatment of myocardial infarction in SD rats to establish a rat modeling, then the rats were grouped according to the different injections, and the heart rhythm variability (HRV) of different groups of rats with myocardial infarction (MI) was recorded. Finally,
the program electrical stimulation (PES) was used to analyze the ventricular effective refractory period (VERP) and ventricular arrhythmia (VAs) in rats. In the experiment, Ag–C/Au nanomaterials produced strong yellow fluorescence under 370 nm ultraviolet excitation, with an approximate
particle size of 1.6 nm; through spectral data fitting, it was found that the recombinant protein ephrin-b2 could be adsorbed on the surface of the composite nanomaterials, making the intensity of the characteristic peak decreased; the myocardial indicators of myocardial infarction rats based
on ephrin- b2 + Ag–C/Au group were significantly improved, while the grouping of rats using Ag–C/Au alone didn't affect the electrophysiological characteristics after myocardial infarction, that is, ephrin-b2 could improve arrhythmia after myocardial infarction, and the improvement
effect would be further strengthened when Ag–C/Au was added.
Background: Currently, vascular endothelial damage caused by hypertension is one of the major health challenges facing countries around the world. Neohesperidin has been shown to play an important role in tumorigenesis and tumorigenesis, cardiac hypertrophy and remodeling, and oxidative stress. However, whether Nehesperidin plays an important role in endothelial injury induced by hypertension has not been clarified.Results: In this study, Angiotensin II was used to induce hypertension in mice. Blood pressure and vasoconstrictor function were measured, vascular thickness and fibrosis were detected by H&E and Masson tricolor staining, vascular inflammation was detected by immunofluorescence, oxidative stress was detected by DHE staining, and markers such as fibrosis, hypertrophy and oxidative stress were detected by qPCR. At the same time, we observed the effect of Nehesperidin on Ang II-induced HUVECs. The results showed that neohesperidin can significantly inhibit Ang II-induced hypertension, vascular thickness, fibrosis, oxidative stress and inflammation in vivo and in vitro. Conclusions: The results suggested that Nehesperidin could act as an antioxidant to significantly inhibit Ang II-induced hypertension and endothelial injury in HUVECs in mice by inhibiting oxidative stress response.
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